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Updated: Feb 15, 2026

RNA Next-Generation Sequencing and a Bioinformatics Pipeline to Identify Expressed LINE-1s at the Locus-Specific Level
Published on: May 19, 2019
Multiplatform next-generation sequencing identifies novel RNA molecules and transcript isoforms of the endogenous
Norbert Moldován1, Attila Szucs1, Dóra Tombácz1,2
1Department of Medical Biology, Faculty of Medicine, University of Szeged, Szeged H-6720, Hungary.
Abstract:
In this study, we applied short- and long-read RNA sequencing techniques, as well as PCR analysis to investigate the transcriptome of the porcine endogenous retrovirus (PERV) expressed from cultured porcine kidney cell line PK-15. This analysis has revealed six novel transcripts and eight transcript isoforms, including five length and three splice variants. We were able to establish whether a deletion in a transcript is the result of the splicing of mRNAs or of genomic deletion in one of the PERV clones. Additionally, we re-annotated the formerly identified RNA molecules. Our analysis revealed a higher complexity of PERV transcriptome than it was earlier believed.
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