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Related Concept Videos

Displacement Current01:19

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Ampère's law, in its usual form, does not work in places where the current changes with time and is not steady. Thus, Maxwell suggested including an additional contribution, called the displacement current, Id, to the real conduction current I.
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Position and Displacement01:31

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The position of an object defines its location relative to a convenient frame of reference at any particular time. A frame of reference is an arbitrary set of axes from which the position and motion of an object are described. Earth is often used as a frame of reference, and we often describe the position of an object as it relates to stationary objects on Earth. For example, a rocket launch could be described in terms of the position of the rocket with respect to Earth as a whole. On the other...
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Significance of Displacement Current01:27

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A displacement current is analogous to a real current in Ampère's law, participating in Ampère's law the same way as the usual conduction current. However, it is produced by a changing electric field. Displacement current is defined in terms of a time-varying electric field, and also has an associated displacement current density. By adding a term accounting for displacement current, Maxwell modified the existing Ampère's law, which is now called generalized Ampère's law.
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Uniform circular motion is motion in a circle at a constant speed. Although this is the simplest case of rotational motion, it is very useful for many situations and is used to introduce rotational variables. When a particle is moving in a circle, the coordinate system is fixed and serves as a frame of reference to define the particle’s position. Its position vector from the origin of the circle to the particle sweeps out the angle θ, which increases in the counterclockwise direction...
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Brain lateralization refers to the division of mental processes and functions between the two hemispheres of the brain, a phenomenon that optimizes neural efficiency and underpins complex abilities in humans. This specialization allows each hemisphere to perform tasks where it has a comparative advantage, facilitating more refined cognitive capabilities across different domains.
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Author Spotlight: Enhancing CAR-T Cell Function in Syngeneic Tumor Models
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Deterministic Lateral Displacement: The Next-Generation CAR T-Cell Processing?

Roberto Campos-González1, Alison M Skelley1, Khushroo Gandhi1

  • 11 GPB Scientific LLC, Richmond, VA, USA.

SLAS Technology
|January 25, 2018
PubMed
Summary
This summary is machine-generated.

Deterministic lateral displacement (DLD) processing of apheresis products yields high cell recovery and a superior T-central memory phenotype, crucial for CAR T cell manufacturing. This method enhances cell expansion and reduces variability compared to traditional techniques.

Keywords:
CAR T cellscell processinggene therapyimmunotherapymicrofluidics

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Area of Science:

  • Biotechnology
  • Cellular immunotherapy
  • Bioprocessing

Background:

  • Reliable cell recovery and expansion are critical for therapeutic cell manufacturing, including chimeric antigen receptor (CAR) T cells.
  • Current methods for processing apheresis products can be variable and may not optimize T cell phenotype for downstream expansion.

Purpose of the Study:

  • To evaluate the efficacy of a novel deterministic lateral displacement (DLD) device for processing apheresis blood products.
  • To compare the cell recovery, platelet depletion, T cell phenotype, and expansion potential of DLD-processed cells against traditional methods.

Main Methods:

  • Manufacturing a high-parallel deterministic lateral displacement (DLD) device with diamond microposts.
  • Processing apheresis blood products using the DLD device.
  • Assessing cell recovery, platelet depletion, T cell phenotype (specifically T-central memory), and cell expansion post-processing.
  • Comparing DLD processing with Ficoll-Hypaque and direct magnetic separation methods.

Main Results:

  • Achieved 80% cell recovery and 87% platelet depletion using the DLD device.
  • DLD-processed T cells exhibited a high conversion to the T-central memory phenotype (>50% with low variation) and superior expansion.
  • Compared to Ficoll and magnetic methods, DLD resulted in twofold greater central memory cells and more consistent phenotype conversion across donors.

Conclusions:

  • Deterministic lateral displacement processing of apheresis products offers a promising approach for therapeutic cell manufacturing.
  • DLD technology enhances T cell recovery, promotes a favorable T-central memory phenotype, and improves expansion potential.
  • The DLD method presents a path towards a simplified, automated, closed system for cell manufacturing, mitigating risks of error and contamination.