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Updated: Jan 7, 2026

Tuning a Parallel Segmented Flow Column and Enabling Multiplexed Detection
Published on: December 15, 2015
Tunable single-column deterministic lateral displacement device by adjustable crossflow.
Miftahul Jannat Rasna1, James C Sturm1
1Department of Electrical and Computer Engineering, Princeton University, Princeton, New Jersey, 08544, USA. mrasna@princeton.edu.
This study introduces a tunable deterministic lateral displacement (DLD) device for size-based micro-particle separation. The novel single-column design offers adjustable critical size and high throughput, overcoming limitations of conventional DLD systems.
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Area of Science:
- Microfluidics
- Biotechnology
- Particle Separation Technology
Background:
- Deterministic lateral displacement (DLD) devices enable high-resolution, size-based micro-particle separation using tilted micro-post arrays.
- Conventional DLD devices lack tunability in critical particle size (Dc), which is fixed by geometry.
- Traditional DLD devices require large footprints due to multiple rows/columns for particle separation, limiting throughput.
Purpose of the Study:
- To develop a novel, tunable single-column deterministic lateral displacement (DLD) device.
- To overcome the fixed critical size limitation of conventional DLD devices.
- To enhance throughput and reduce device area for micro-particle separation.
Main Methods:
- A single-column DLD device with 8 bumping obstacles was designed and fabricated.
- Tunability of critical size (Dc) was achieved by adjusting perpendicular crossflow.
- Device performance was evaluated for separation efficiency and throughput/area.
Main Results:
- Demonstrated tunability of critical size (Dc) from below 5 μm to above 10 μm within a single device.
- Achieved high separation efficiency of approximately 99.9%.
- Obtained a high throughput/area of 45 μL min⁻¹ mm⁻².
- Noted resolution degradation at high flow rates (Re > 10) due to 3D flow patterns.
Conclusions:
- The novel tunable single-column DLD device offers a significant advancement over conventional designs.
- This technology provides a compact and adaptable solution for precise micro-particle separation.
- Further research may focus on optimizing performance at higher flow rates.

