An Accessible Organotypic Microvessel Model Using iPSC-Derived Endothelium.

Area of Science:

• Biotechnology
• Stem Cell Biology
• Vascular Biology

Background:

• Organotypic models offer increased physiological relevance but often overlook cell source.
• Induced pluripotent stem cells (iPSCs) provide a more relevant and less variable cell source compared to cell lines or primary cells.
• Advancements have made iPSC-derived cells commercially available at costs comparable to traditional cell lines.

Purpose of the Study:

• To demonstrate the use of iPSC-derived endothelium for generating a functional microvessel model.
• To leverage high-precision structural and microenvironmental control with iPSC advantages for in vitro endothelial biology modeling.
• To create improved, accessible in vitro models for tissue, disease, and patient-specific research.

Main Methods:

• Utilizing induced pluripotent stem cell (iPSC)-derived endothelial cells.
• Employing a design approach with high-precision structural and microenvironmental control.
• Generating and characterizing functional microvessel models in vitro.

Main Results:

• iPSC microvessels exhibited endothelial characteristics, barrier function, and secretion of angiogenic/inflammatory mediators.
• The microvessels responded to extracellular microenvironment changes by altering their phenotype.
• In immune studies, iPSC endothelial vessels facilitated neutrophil extravasation and migration.

Conclusions:

• iPSC-derived endothelium is suitable for creating functional in vitro microvessel models.
• Combining iPSC cell sources with organotypic models enhances in vitro modeling capabilities.
• This approach advances the development of accessible, physiologically relevant in vitro tissue, disease, and patient-specific models.