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Porcine IFI30 inhibits PRRSV proliferation and host cell apoptosis in vitro

Jia Guo1, Mengjiao Zhou1, Xin Liu1

  • 1College of Animal Science, Jilin University, Xi An Road 5333, Changchun, Jilin 130062, PR China.

Gene
|January 26, 2018
PubMed

Insights

Porcine interferon-gamma-inducible protein 30 (IFI30) expression inhibits Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) transcription and M protein production. IFI30 also modulates host cell apoptosis in PRRSV-infected cells.

Area of Science:

  • Immunology
  • Virology
  • Cell Biology

Background:

  • Interferon-gamma-inducible protein 30 (IFI30) is crucial for antigen processing in adaptive immunity.
  • Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) poses a significant threat to swine health and the global pork industry.
  • Understanding host-pathogen interactions is key to developing effective antiviral strategies.

Purpose of the Study:

  • To investigate the role of porcine IFI30 in regulating PRRSV proliferation.
  • To determine the impact of IFI30 expression on PRRSV transcription and protein production.
  • To assess the effect of IFI30 on host cell apoptosis during PRRSV infection.

Main Methods:

  • Transfection of MARC-145 and pig Sertoli (ST) cells with porcine IFI30 expression vectors.
  • PRRSV infection of transfected cells.
  • Absolute real-time quantitative PCR to quantify PRRSV copy numbers.
  • Western blot analysis for PRRSV M protein detection.
  • Flow cytometry to assess host cell apoptosis.

Main Results:

  • Porcine IFI30 expression significantly inhibited PRRSV transcription.
  • IFI30 expression reduced the production of PRRSV M protein.
  • IFI30 expression decreased apoptosis in MARC-145 cells.
  • IFI30 knockdown increased apoptosis in porcine ST cells, while overexpression did not.

Conclusions:

  • Porcine IFI30 expression demonstrates an inhibitory effect on PRRSV proliferation in vitro.
  • IFI30 plays a role in modulating host cell apoptosis in response to PRRSV infection.
  • These findings suggest IFI30 as a potential target for controlling PRRSV.
Keywords:
IFI30MARC-145 cellsPRRSVProliferationST cells

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