Isolation, identification and characterization of novel Bacillus subtilis

Area of Science:

• Microbiology
• Bacteriology

Background:

• Staphylococcus aureus poses a significant threat due to its increasing antibiotic resistance.
• Identifying novel antibacterial agents is crucial for combating infectious diseases.

Purpose of the Study:

• To isolate and characterize a novel bacterium with antibacterial properties.
• To evaluate the antibacterial spectrum of the identified bacterium and its products.

Main Methods:

• Bacterial isolation, culture, and morphological/biochemical characterization.
• 16S rRNA gene sequencing for identification and homology analysis.
• Antibacterial activity testing against various pathogens using crude bacteriocin.

Main Results:

• A Gram-positive, spore-forming Bacillus species was isolated and identified as Bacillus subtilis with 99.4% homology.
• This Bacillus subtilis strain demonstrated inhibitory effects against Staphylococcus aureus, Escherichia coli, Enterococcus, and Salmonella.
• The crude bacteriocin produced by the bacterium was effective against the tested pathogens.

Conclusions:

• The identified Bacillus subtilis strain is a promising source of novel antibacterial compounds.
• Bacteriocin from this strain has potential applications in controlling Gram-positive and Gram-negative bacterial infections.