Subchronic Oral Dose Toxicity Study of Enterococcus Faecalis 2001 (EF 2001) in Mice

Area of Science:

• Microbiology
• Toxicology
• Preventive Medicine

Background:

• Enterococcus Faecalis 2001 (EF 2001) is a heat-treated bacterium used in preventive medicine.
• General toxicity studies are essential to ensure the safety of such products.

Purpose of the Study:

• To evaluate the general toxicity of EF 2001 following single and repeated oral administrations in mice.
• To determine the safety of EF 2001 for potential application in preventive medicine.

Main Methods:

• Male and female ICR mice received EF 2001 orally via gavage at doses of 1,000, 3,000, or 5,000 mg/kg/day for 13 weeks.
• Distilled water served as the control.
• Evaluations included general condition observation, body weight, food and water consumption, blood tests, urinalysis, and pathological examinations.

Main Results:

• No significant differences were observed between the EF 2001 and control groups in any of the measured parameters.
• No abnormal symptoms or deaths occurred during the study period.
• The 50% lethal dose (LD50) was estimated to be greater than 5,000 mg/kg body weight/day for both sexes.

Conclusions:

• EF 2001 demonstrates no notable toxicity upon single and repeated oral administration in mice.
• The product is considered safe for oral ingestion and has potential for use in preventive medicine.
• EF 2001 is speculated to not adversely affect living organisms due to a lack of cellular and organ toxicity.