Detection of Abrin by Electrochemiluminescence Biosensor Based on Screen Printed Electrode

Area of Science:

• Analytical Chemistry
• Biosensor Technology
• Environmental Monitoring

Background:

• Traditional methods for abrin detection are often time-consuming and not suitable for field use.
• There is a need for rapid, sensitive, and portable detection systems for abrin in outdoor environments.

Purpose of the Study:

• To develop a portable electrochemiluminescence (ECL) biosensor for the in situ detection of abrin.
• To achieve high sensitivity, specificity, and simplified operation for abrin analysis.

Main Methods:

• Fabrication of a portable ECL biosensor using screen-printed electrodes.
• Immobilization of polyclonal antibody (pcAb) on magnetic microspheres via biotin-avidin method.
• Utilized Ru(bpy)₃²⁺-labeled monoclonal antibody (mcAb) as signal probe for sandwich assay.

Main Results:

• Achieved a linear detection range of 0.5-1000 ng/mL for abrin.
• Established a regression equation (Y = 89.251lgX + 104.978, R = 0.9989).
• Determined a limit of detection (LOD) of 0.1 ng/mL with high sensitivity, specificity, and anti-interference ability.

Conclusions:

• The developed portable ECL biosensor is suitable for trace abrin detection in various environmental samples.
• The biosensor offers good recovery and reproducibility, outperforming traditional devices in portability.
• Miniaturization and portability enable potential for on-site abrin monitoring requirements.