Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Fibril-associated Collagen01:11

Fibril-associated Collagen

3.4K
Fibril-associated collagens are a type of collagens present in the extracellular matrix with interrupted triple helices or FACIT (Fibril-associated collagens interrupted triple-helices). FACIT help connect and attach the collagen fibrils with each other as well as with other proteins of the extracellular matrix.
For example, the type II collagen fibrils in cartilage have covalently bound type IX fibril-associated collagens at regular intervals. Other types of fibril-associated collagens are...
3.4K
Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

7.6K
Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such...
7.6K
Collagens are the Major Structural Proteins of ECM01:13

Collagens are the Major Structural Proteins of ECM

5.9K
Three main types of fibers are secreted by fibroblasts: collagen fibers, elastic fibers, and reticular fibers. Collagen fiber is made from fibrous protein subunits linked together to form a long, straight fiber. Collagen fibers, while flexible, have great tensile strength, resist stretching, and give ligaments and tendons their characteristic resilience and strength. These fibers hold connective tissues together, even during the body's movement.
Connective tissue proper includes loose...
5.9K
Type IV Collagen of Basal Lamina01:05

Type IV Collagen of Basal Lamina

3.1K
Type IV collagen is a 400 nm long, network-forming collagen that acts as a barrier between the epithelial and endothelial cells. Type IV collagen  forms the backbone of the basement membrane by scaffolding with laminin, entactin, proteoglycans, and fibronectin. Apart from rendering structural support to the basement membrane, it also helps entail signaling potentials necessary for both pathological and physiological functions.
A type IV collagen molecule has six alpha chains which can...
3.1K
DNA Agarose Gel Electrophoresis02:35

DNA Agarose Gel Electrophoresis

115.1K
Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
115.1K
Silica Gel Column Chromatography: Overview01:10

Silica Gel Column Chromatography: Overview

3.7K
Silica gel column chromatography is a technique for separating compounds using a column packed with silica gel as the stationary phase. This method relies on differences in the polarity of compounds. Based on their polarities, compounds move between the stationary phase (silica gel) and the mobile phase (the solvent), forming discrete bands in the column.
Polar components tend to bind strongly to the silica gel, causing them to move slowly through the column. In contrast, nonpolar compounds...
3.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Gold-nanoparticle decorated allograft tendons confer mitigation of inflammatory and oxidative species in vitro.

Journal of biomaterials applications·2025
Same author

Application of the dual-model interpretability framework of XGBoost-SHAP and GAT-GNNExplainer to investigate the impact of the built environment on traffic accidents at intersections.

Accident; analysis and prevention·2025
Same author

Concomitant ileocolic intussusception and perforated appendicitis in a toddler.

BMJ case reports·2025
Same author

Influence of road environmental factors on traffic accidents involving vulnerable road users through negative binomial models.

PloS one·2025
Same author

Incidental GIST in Patients Undergoing Bariatric Surgery: A Systematic Review of Incidence and Management.

Obesity surgery·2025
Same author

Investigation of Liquid Collagen Ink for Three-Dimensional Printing.

Micromachines·2024

Related Experiment Video

Updated: Feb 15, 2026

Gold Nanoparticle Synthesis
13:42

Gold Nanoparticle Synthesis

Published on: July 10, 2021

15.9K

Gold Nanoparticle-Collagen Gels for Soft Tissue Augmentation.

Sheila A Grant1, Jiaxun Zhu1, Jonathan Gootee1

  • 11 Department of Bioengineering, College of Engineering, University of Missouri , Columbia, Missouri.

Tissue Engineering. Part A
|January 30, 2018
PubMed
Summary

Gold nanoparticles (AuNPs) conjugated to collagen may improve soft tissue filler longevity and biocompatibility. This study found AuNP-collagen fillers showed enhanced duration and low irritation in animal models.

Keywords:
collagendermalgold nanoparticleshyaluronic acid

More Related Videos

Fabrication of Micro-tissues using Modules of Collagen Gel Containing Cells
09:28

Fabrication of Micro-tissues using Modules of Collagen Gel Containing Cells

Published on: December 13, 2010

13.3K
Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration
09:23

Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration

Published on: June 16, 2015

21.5K

Related Experiment Videos

Last Updated: Feb 15, 2026

Gold Nanoparticle Synthesis
13:42

Gold Nanoparticle Synthesis

Published on: July 10, 2021

15.9K
Fabrication of Micro-tissues using Modules of Collagen Gel Containing Cells
09:28

Fabrication of Micro-tissues using Modules of Collagen Gel Containing Cells

Published on: December 13, 2010

13.3K
Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration
09:23

Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration

Published on: June 16, 2015

21.5K

Area of Science:

  • Biomaterials science
  • Tissue engineering
  • Nanotechnology

Background:

  • Collagen soft tissue fillers have limited longevity due to rapid reabsorption.
  • Extensive cross-linking improves longevity but negatively impacts microstructure and biomechanics.

Purpose of the Study:

  • To enhance the longevity of collagen fillers while maintaining a natural microstructure.
  • To develop an injectable collagen filler stabilized with gold nanoparticles (AuNPs).

Main Methods:

  • Conjugating AuNPs to fibrilized collagen to create an injectable filler.
  • Conducting a long-term animal study in Yucatan swine.
  • Comparing AuNP-collagen formulations against cross-linked collagen and hyaluronic acid (HA).

Main Results:

  • AuNP-collagen demonstrated enhanced longevity over 6 months compared to HA and cross-linked collagen.
  • The AuNP-collagen construct showed low irritation scores.
  • Histology confirmed good biocompatibility of the AuNP-collagen filler.

Conclusions:

  • Gold nanoparticles can stabilize collagen constructs, increasing longevity.
  • AuNP-collagen fillers offer a promising alternative for soft tissue augmentation.
  • This approach may overcome the limitations of current collagen-based fillers.