Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Determining the pH of Salt Solutions

Determining the pH of Salt Solutions

The pH of a salt solution is determined by its component anions and cations. Salts that contain pH-neutral anions and the hydronium ion-producing cations form a solution with a pH less than 7. For example, in ammonium nitrate (NH4NO3) solution, NO3− ions do not react with water whereas NH4+ ions produce the hydronium ions resulting in the acidic solution. In contrast, salts that contain pH-neutral cations and the hydroxide ion-producing anions form a solution with a pH greater than 7. For...

One-Compartment Open Model: Urinary Excretion Data and Determination of k

One-Compartment Open Model: Urinary Excretion Data and Determination of k

The one-compartment open model leverages urinary excretion data to estimate renal clearance, which gauges the kidney's capacity to expel a drug. This method offers several benefits, including directly measuring drug elimination and assessing the kidney's contribution to overall drug clearance. However, this approach has limitations. It assumes sole renal excretion of the drug, which is not true for all drugs. Accurate urinary excretion and plasma drug concentration measurement can also...

Electron Behavior

Electron Behavior

Overview
Electrons are negatively charged subatomic particles that are attracted to an orbit around the positively-charged nucleus of an atom. They reside in locations that are associated with energy levels called shells and are further organized into sub-shells and orbitals within each shell.
Electrons Orbit the Nucleus
Electrons are found in specific locations outside of the nucleus. The shell in which an electron resides indicates the general energy level of the electron: those closer to the...

Scatter Plot

Scatter Plot

The most common and easiest way to display the relationship between two variables, x and y, is a scatter plot. A scatter plot shows the direction of a relationship between the variables. A clear direction happens when there is either:

Chemical Reactions in Aqueous Solutions

Chemical Reactions in Aqueous Solutions

Chemical substances interact in many different ways. Certain chemical reactions exhibit common patterns of reactivity. Due to the vast number of chemical reactions, it becomes necessary to classify them based on the observed patterns of interaction.

Expressing Solution Concentration

Expressing Solution Concentration

A solute is a component of a solution that is typically present at a much lower concentration than the solvent. Solute concentrations are often described with qualitative terms such as dilute (of relatively low concentration) and concentrated (of relatively high concentration).
Concentrations may be quantitatively assessed using a wide variety of measurement units, each convenient for particular applications. Molarity (M) is a useful concentration unit for many applications in chemistry.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Structure of a Stand-Alone Homodimeric NRPS Condensation Domain Reveals Occlusion of the Canonical Carrier-Protein Interface.

bioRxiv : the preprint server for biology·2026

Same author

Minimized sample consumption for time-resolved serial crystallography applied to the redox cycle of human NQO1.

Communications chemistry·2026

Same author

Structure Characterization of Bacterial Microcompartment Shells via X-ray Scattering and Coordinate Modeling: Evidence for Adventitious Capture of Cytoplasmic Proteins.

ACS applied bio materials·2025

Same author

RNA structures and dynamics with Å resolution revealed by x-ray free-electron lasers.

Science advances·2023

Same author

Detection of a Geminate Photoproduct of Bovine Cytochrome <i>c</i> Oxidase by Time-Resolved Serial Femtosecond Crystallography.

Journal of the American Chemical Society·2023

Same author

Synchronous RNA conformational changes trigger ordered phase transitions in crystals.

Nature communications·2021

Same journal

Efficient evidence-based genome annotation with EviAnn.

Nature methods·2026

Same journal

ClairS: a deep-learning method for long-read tumor-normal pair somatic small variant calling.

Nature methods·2026

Same journal

RNAbpFlow: base pair-augmented SE(3) flow matching for conditional RNA 3D structure generation.

Nature methods·2026

Same journal

Spatio-DARLIN enables robust and efficient in situ lineage tracing in mice at single-cell resolution.

Nature methods·2026

Same journal

EasyGrid: a versatile platform for automated cryo-EM sample preparation and quality control.

Nature methods·2026

Same journal

Cloud-based microscope enables live neuroimaging for 24 h and beyond with worldwide access.

Nature methods·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Feb 15, 2026

Contrast-Matching Detergent in Small-Angle Neutron Scattering Experiments for Membrane Protein Structural Analysis and Ab Initio Modeling

Contrast-Matching Detergent in Small-Angle Neutron Scattering Experiments for Membrane Protein Structural Analysis and Ab Initio Modeling

Published on: October 21, 2018

Ab initio electron density determination directly from solution scattering data.

Thomas D Grant^1,2

¹Department of Structural Biology, University at Buffalo, State University of New York, New York, USA.

|January 30, 2018

Summary

This summary is machine-generated.

A new iterative algorithm directly calculates electron density from solution scattering data, bypassing traditional modeling. This method enhances accuracy and resolution for diverse biological macromolecules.

More Related Videos

Computation of Atmospheric Concentrations of Molecular Clusters from ab initio Thermochemistry

Computation of Atmospheric Concentrations of Molecular Clusters from ab initio Thermochemistry

Published on: April 8, 2020

Analyzing Melts and Fluids from Ab Initio Molecular Dynamics Simulations with the UMD Package

Analyzing Melts and Fluids from Ab Initio Molecular Dynamics Simulations with the UMD Package

Published on: September 17, 2021

Related Experiment Videos

Last Updated: Feb 15, 2026

Contrast-Matching Detergent in Small-Angle Neutron Scattering Experiments for Membrane Protein Structural Analysis and Ab Initio Modeling

Contrast-Matching Detergent in Small-Angle Neutron Scattering Experiments for Membrane Protein Structural Analysis and Ab Initio Modeling

Published on: October 21, 2018

Computation of Atmospheric Concentrations of Molecular Clusters from ab initio Thermochemistry

Computation of Atmospheric Concentrations of Molecular Clusters from ab initio Thermochemistry

Published on: April 8, 2020

Analyzing Melts and Fluids from Ab Initio Molecular Dynamics Simulations with the UMD Package

Analyzing Melts and Fluids from Ab Initio Molecular Dynamics Simulations with the UMD Package

Published on: September 17, 2021

Area of Science:

Structural biology
Biophysics
Biochemistry

Background:

Solution scattering techniques provide low-resolution structural information about molecules in solution.
Current methods often rely on modeling, which introduces assumptions and limitations.
Accurate electron density maps are crucial for understanding molecular structure and function.

Purpose of the Study:

To develop and validate a novel algorithm for direct electron density calculation from solution scattering data.
To overcome limitations associated with traditional modeling approaches.
To provide a versatile tool applicable to various molecular systems.

Main Methods:

Development of a novel iterative structure factor retrieval algorithm.
Direct calculation of electron density without prior structural models.
Validation using experimental solution scattering data from 12 diverse biological macromolecules.

Main Results:

Demonstrated direct calculation of electron density from solution scattering data.
Successfully validated the algorithm across 12 different biological macromolecules.
The method avoids assumptions inherent in modeling, improving accuracy and resolution.

Conclusions:

The novel algorithm enables direct electron density calculation from solution scattering data.
This approach offers improved accuracy and resolution compared to modeling.
The algorithm is broadly applicable to a wide range of molecular systems in structural biology.