ProtDataTherm: A database for thermostability analysis and engineering of proteins
Hassan Pezeshgi Modarres1,2,3, Mohammad R Mofrad2,4, Amir Sanati-Nezhad1,5
1BioMEMS and Bioinspired Microfluidic Laboratory, Department of Mechanical and Manufacturing Engineering, University of Calgary, Calgary, Alberta, Canada.
Plos One
|January 30, 2018
Summary
Protein thermostability engineering benefits from categorized data. The new ProtDataTherm database provides over 14 million protein sequences, aiding in protein stability prediction and engineering.
Area of Science:
- Biochemistry
- Structural Biology
- Bioinformatics
Background:
- Protein thermostability engineering enhances protein resistance to high temperatures, broadening applications.
- Efficient engineering requires diverse, thermostability-classified data sources (sequences, structures) for various protein families.
- Existing data sources lack easy accessibility for such comprehensive data.
Purpose of the Study:
- Introduce the ProtDataTherm database, the first release for protein thermostability analysis and engineering.
- Provide a comprehensive resource of over 14 million protein sequences categorized by thermal stability and protein family.
- Facilitate a deeper understanding of protein thermostability and stability engineering.
Main Methods:
- Compiled and categorized over 14 million protein sequences based on thermal stability (psychrophilic, mesophilic, thermophilic) and protein family.
- Integrated sequence and 3D structure data for enhanced analysis.
- Developed a proof-of-concept to suggest thermostability-improving mutations using database data and ProTherm database information.
Main Results:
- Launched ProtDataTherm, a novel database containing over 14 million categorized protein sequences.
- The database categorizes proteins as psychrophilic, mesophilic, and thermophilic, aiding in stability prediction.
- Demonstrated the utility of the database by identifying potential thermostability-enhancing mutations for a sample protein.
Conclusions:
- ProtDataTherm is a valuable resource for protein thermostability research and engineering.
- The categorized data facilitates the development of new protein stability prediction tools.
- This database supports advancements in understanding and manipulating protein thermal stability.
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