Related Experiment Video

Updated: Feb 15, 2026

Lateral Fluid Percussion: Model of Traumatic Brain Injury in Mice
11:04

Lateral Fluid Percussion: Model of Traumatic Brain Injury in Mice

Published on: August 22, 2011

41.5K

Sinomenine reduces neuronal cell apoptosis in mice after traumatic brain injury via its effect on mitochondrial

Chuanjing Fu1, Qi Wang2, Xiaofu Zhai3

  • 1Department of Neurosurgery, Jiangsu Hospital of Traditional Chinese Medicine, Nanjing.

Drug Design, Development and Therapy
|January 31, 2018
PubMed
Abstract

Insights

Sinomenine (SIN) protects brain cells after traumatic brain injury (TBI) by reducing cell death. This study investigated SIN's mechanisms, finding it inhibits apoptosis and protects neurons.

Area of Science:

  • Neuroscience
  • Pharmacology

Background:

  • Traumatic brain injury (TBI) can cause significant brain damage.
  • Sinomenine (SIN) shows potential neuroprotective effects, but its mechanisms are not fully understood.

Purpose of the Study:

  • To investigate the underlying mechanisms of SIN's neuroprotective effects in a TBI model.
  • To elucidate SIN's role in mitigating neuronal damage and apoptosis post-TBI.

Main Methods:

  • A weight-drop TBI model was established in ICR mice.
  • Mice received intraperitoneal SIN or vehicle 30 minutes post-TBI.
  • Evaluated neurological scores, brain water content, neuronal cell death (TUNEL assay), and specific protein expressions (cytochrome c, caspase-3, Bax, MDA, GPx, SOD).

Main Results:

  • SIN treatment partially restored levels of cytochrome c, MDA, GPx, and SOD.
  • SIN significantly reduced caspase-3 expression and TUNEL-positive cells, indicating decreased apoptosis.
  • SIN treatment inhibited Bax translocation to mitochondria and cytochrome c release, improving neuronal survival.

Conclusions:

  • SIN demonstrates neuroprotective effects against TBI by inhibiting mitochondrial-mediated apoptosis.
  • The findings suggest SIN is a promising therapeutic agent for TBI treatment.
Keywords:
apoptosismitochondriasinomeninetraumatic brain injury

More Related Videos

Impulsive Pressurization of Neuronal Cells for Traumatic Brain Injury Study09:24

Impulsive Pressurization of Neuronal Cells for Traumatic Brain Injury Study

Published on: October 12, 2011

10.6K
Electromagnetic Controlled Closed-Head Model of Mild Traumatic Brain Injury in Mice09:07

Electromagnetic Controlled Closed-Head Model of Mild Traumatic Brain Injury in Mice

Published on: September 28, 2022

2.8K

Related Concept Videos

Apoptosis01:30

Apoptosis

Apoptosis is a combination of two Greek words, 'apo' and 'ptosis,' meaning separation and falling off, respectively. Hippocrates used this word to describe gangrene, which was caused due to bandaging of fractured bones. Apoptosis was distinguished from necrosis in 1970 when John Kerr reported observations of morphological changes occurring during apoptosis. During one experiment, he observed that the disruption of blood supply to the liver tissue resulted in a size...
15.3K
Animal Mitochondrial Genetics02:59

Animal Mitochondrial Genetics

Among all the organelles in an animal cell, only mitochondria have their own independent genomes. Animal mitochondrial DNA is a double-stranded, closed-circular molecule with around 20,000 base pairs. Mitochondrial DNA is unique in that one of its two strands, the heavy, or H, -strand is guanine rich, whereas the complementary strand is cytosine rich and called the light, or L, -strand. Compared to nuclear DNA, mitochondrial DNA has a very low percentage of non-coding regions and is marked by...
9.3K
Export of Mitochondrial and Chloroplast Genes02:19

Export of Mitochondrial and Chloroplast Genes

A eukaryotic cell can have up to three different types of genetic systems: nuclear, mitochondrial, and chloroplast. During evolution, organelles have exported many genes to the nucleus; this transfer is still ongoing in some plant species. Approximately 18% of the Arabidopsis thaliana nuclear genome is thought to be derived from the chloroplast’s cyanobacterial ancestor, and around 75% of the yeast genome derived from the mitochondria’s bacterial ancestor. This export has occurred...
4.2K
Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes02:16

Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes

The present-day mitochondrial and chloroplast genomes have retained some of the characteristics of their ancestral prokaryotes and also have acquired new attributes during their evolution within eukaryotic cells. Like prokaryotic genomes, mitochondrial and chloroplast genomes neither bind with histone-like proteins nor show complex packaging into chromosome-like structures, as observed in eukaryotes. Unlike mitotic cell divisions observed in eukaryotic cells, mitochondria and chloroplasts...
17.1K
Neurons as Communicators of the Brain01:22

Neurons as Communicators of the Brain

Neurons, the fundamental units of the brain and nervous system, function as the primary transmitters of information throughout the body. Their ability to communicate through electrical and chemical signals is vital for every bodily function, from regulating the heartbeat to processing complex thoughts. Each neuron has three main components: the cell body (soma), dendrites, and an axon, each specialized to facilitate swift and efficient neural communication.
Cell Body
The cell body, also known...
3.5K
C4 Pathway and CAM01:27

C4 Pathway and CAM

Most plants use the C3 pathway for carbon fixation. However, some plants, such as sugar cane, corn, and cacti that grow in hot conditions, use alternative pathways to fix carbon and conserve energy loss due to photorespiration. Photorespiration is the process that occurs when the oxygen concentration is high. Under such conditions, the rubisco enzyme in the Calvin cycle binds O2 instead of CO2, which halts photosynthesis and consumes energy.
C4 Pathway
The C4 pathway is used by plants such as...
49.4K