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Updated: Feb 15, 2026

Dissection and Coronal Slice Preparation of Developing Mouse Pituitary Gland
Published on: November 16, 2017
Mass Spectrometry Profiling of Pituitary Glands
Divya Krishnamurthy1, Hassan Rahmoune1, Paul C Guest2
1Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, UK.
Abstract:
Many chronic diseases are associated with hypothalamic-pituitary-adrenal axis dysfunction. Therefore, proteomic profiling of the pituitary gland has potential to uncover new information on the underlying pathways affected in these conditions. This could lead to identification of new biomarkers or drug targets for development of novel therapeutics. Here we present a protocol for preparation of pituitary protein extracts and analysis of the major hormones and accessory proteins using liquid chromatography tandem mass spectrometry (LC-MS/MS). The same methods can be applied in the study of other tissues of the diffuse neuroendocrine system.
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