[Highly expressed CHAF1A and PCNA are positively associated with malignancy of cervical squamous cell carcinoma]

Objective To investigate the expressions of chromatin assembly factor 1 subunit A (CHAF1A) and proliferating cell nuclear antigen (PCNA) in cervical squamous cell carcinoma (CSCC) and their clinical implication. Methods Real-time quantitative PCR was performed to detect the mRNA levels of CHAF1A and PCNA in CSCC and corresponding paracancerous tissues. Immunohistochemistry was used to detect the expressions of CHAF1A and PCNA proteins in normal cervical epithelium tissues (NC), cervical intraepithelial neoplasia tissues (CIN), and CSCC. The correlation between CHAF1A and PCNA expressions and the relationship of the two proteins to the clinical pathological features of cervical cancer were analyzed. Results The expressions of CHAF1A and PCNA in CIN and CSCC were significantly higher than those in NC. In addition, CHAF1A and PCNA expressions were positively correlated in the CSCC tissues. Furthermore, overexpression of CHAF1A was significantly associated with the degree of differentiation, tumor size, depth of cancer invasion and HPV infection. Finally, the up-regulated expression of PCNA was correlated with the degree of differentiation, International Federation of Gynecology and Obstetrics (FIGO) grade, lymph node metastasis, depth of cancer invasion and HPV infection. Conclusion CHAF1A and PCNA are highly expressed in CSCC and associated with the malignancy.