Disseminating Metaproteomic Informatics Capabilities and Knowledge Using the Galaxy-P Framework

Area of Science:

• Microbiology
• Bioinformatics
• Proteomics

Background:

• The microbiome's impact on health and environment is increasingly recognized.
• Metaproteomics is crucial for understanding microbiome functions and host interactions.
• Sophisticated bioinformatics tools are needed for analyzing complex metaproteomic data.

Purpose of the Study:

• To develop and disseminate accessible metaproteomics software and workflows.
• To train researchers in using these bioinformatics resources for microbiome studies.
• To foster community-based development of metaproteomics tools.

Main Methods:

• Developed modular workflows for database generation, peptide spectral matching, taxonomic, and functional analysis.
• Utilized the Galaxy for proteomics (Galaxy-P) platform for web-based dissemination.
• Selected and packaged software through a community "Contribution Fest".

Main Results:

• Created easily accessible and documented metaproteomics software tools and workflows.
• Resources are available on GitHub, Galaxy Toolshed, and a public Galaxy instance.
• Workflows are suitable for training novice researchers via online resources and workshops.

Conclusions:

• The Galaxy-P platform provides essential metaproteomics informatics resources.
• These tools increase access to and utilization of metaproteomics software.
• The community-driven approach facilitates ongoing development and dissemination of tools.