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Related Concept Videos

MicroRNAs01:22

MicroRNAs

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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Regulation of Expression Occurs at Multiple Steps02:24

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Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
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Frequency-dependent Selection01:21

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When the fitness of a trait is influenced by how common it is (i.e., its frequency) relative to different traits within a population, this is referred to as frequency-dependent selection. Frequency-dependent selection may occur between species or within a single species. This type of selection can either be positive—with more common phenotypes having higher fitness—or negative, with rarer phenotypes conferring increased fitness.
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Electric Potential and Potential Difference01:16

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Suppose a positive test charge moves away from a positive static charge, then the Coulomb force does positive work, and its electric potential energy decreases. The potential energy per unit charge is defined as the electric potential. The electric potential is independent of the test charge.
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Difference from Background: Limit of Detection01:05

Difference from Background: Limit of Detection

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The limit of detection (LOD) is the smallest amount of analyte that can be distinguished from the background noise. The LOD value corresponds to the concentration at which the analyte signal is three times larger than the standard deviation of the blank signal. Below this value, the analyte signal cannot be differentiated from the background noise. It is calculated by dividing the calibration slope by 3 times the standard deviation of the blank signals.
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Noninvasive Monitoring of Lesion Size in a Heterologous Mouse Model of Endometriosis
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MicroRNA expression pattern differs depending on endometriosis lesion type.

Maria E Haikalis1, Jocelyn M Wessels1, Nicholas A Leyland1

  • 1Department of Obstetrics & Gynecology, McMaster University, Hamilton, Ontario, Canada.

Biology of Reproduction
|February 1, 2018
PubMed
Summary
This summary is machine-generated.

MicroRNA (miRNA) expression differs in the endometrium of women with endometriosis and varies by lesion type. This finding may help personalize care for endometriosis patients.

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Area of Science:

  • Reproductive biology
  • Molecular genetics
  • Biochemistry

Background:

  • Endometriosis is associated with differential microRNA (miRNA) expression in eutopic endometrium and endometriotic lesions.
  • Endometriotic lesions exhibit biochemical distinctness, suggesting varying miRNA expression profiles across lesion types (endometriomas, peritoneal, deep-infiltrating).

Purpose of the Study:

  • To investigate differential miRNA expression in eutopic endometrium and various endometriotic lesion types.
  • To determine if miRNA expression patterns correlate with specific endometriotic lesion types.

Main Methods:

  • Quantitative real-time polymerase chain reaction (qRT-PCR) was used to analyze the expression of specific miRNAs (miR-9, miR-21, miR-424, miR-10a, miR-10b, miR-204).
  • Samples included endometrial biopsies and ectopic implants from 38 women undergoing surgery for endometriosis.
  • Analysis focused on distinct lesion types: endometriomas, peritoneal, and deep-infiltrating implants.

Main Results:

  • miR-204 expression was significantly lower in the eutopic endometrium of women with endometriosis compared to controls.
  • Relative expression of miR-21, miR-424, and miR-10b varied significantly across different endometriotic lesion types.
  • All studied miRNAs showed differential expression between endometriotic lesions and matched eutopic endometrium.

Conclusions:

  • miRNA expression in the eutopic endometrium is altered in women with endometriosis compared to controls.
  • miRNA expression patterns are dependent on the specific type of endometriotic lesion.
  • Identifying distinct miRNA profiles for different lesion types could aid in developing individualized treatment strategies for endometriosis.