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Updated: Aug 9, 2026
12:37
Pharmacologic Induction of Epidermal Melanin and Protection Against Sunburn in a Humanized Mouse Model
Published on: September 7, 2013
[Mouse melanoma antigen immunological properties, structure and genes]
Gan to Kagaku Ryoho. Cancer & Chemotherapy
|March 1, 1986
Summary
Researchers identified melanoma antigens, including GM3 and associated proteins, and cloned the genomic DNA responsible for their expression. This advance aids in understanding melanoma immunology and developing targeted therapies.
Area of Science:
- Immunology
- Molecular Biology
- Biochemistry
Context:
- B16 melanoma cells were used to analyze melanoma antigen properties.
- Monoclonal antibodies M2590 and M562 were employed to characterize antigen composition.
Purpose:
- To identify and characterize melanoma antigens.
- To clone the genomic DNA encoding melanoma antigens.
- To investigate the role of melanoma antigens in immune responses.
Summary:
- Melanoma antigens were found to be composed of GM3 (NeuAc) and associated proteins (31K, 58K, 80K).
- Antibody M2590 detected GM3, while M562 recognized a protein determinant.
- Genomic DNA coding for melanoma antigens was cloned from B16 melanoma cells using cosmid libraries and transfection into human melanoma cells.
- The clone pD2-7 reproducibly expressed M562 determinants in human melanoma cells.
Impact:
- Provides a molecular basis for understanding melanoma antigen recognition by T cells.
- Facilitates the development of novel diagnostic and therapeutic strategies for melanoma.
- Advances the field of cancer immunology and immunotherapy research.
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