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Extracellular RNA Isolation from Cell Culture Supernatant
Sawen Bakr1, Bridget Simonson1, Kirsty M Danielson2,1
1Cardiovascular Research Center, Massachusetts General Hospital, Boston, MA, USA.
Methods in Molecular Biology (Clifton, N.J.)
|February 2, 2018
Summary
Researchers present standardized protocols for isolating extracellular RNAs (exRNAs) from cell culture supernatants. These methods use readily available kits, aiding the study of RNA biomarkers and intercellular communication.
Area of Science:
- Biochemistry
- Molecular Biology
- Genomics
Background:
- Extracellular RNAs (exRNAs) are increasingly recognized as crucial molecules in intercellular communication.
- Investigating exRNAs requires efficient isolation methods from biological samples like cell culture supernatants.
- Existing RNA isolation techniques vary, necessitating standardized protocols for reproducibility.
Purpose of the Study:
- To describe standardized protocols for isolating extracellular RNAs from cell culture supernatants.
- To provide researchers with reproducible methods using commercially available kits.
- To facilitate the study of exRNAs as biomarkers and mediators of cellular communication.
Main Methods:
- Standardized protocols were developed for RNA isolation from cell culture supernatants.
- Commercially available kits and reagents were utilized in the described protocols.
- Protocols focus on efficiency and reproducibility for extracellular RNA extraction.
Main Results:
- The study successfully outlines several standardized protocols for exRNA isolation.
- The described methods are accessible, employing common commercial kits.
- These protocols enable reliable extraction of extracellular RNAs from cell culture media.
Conclusions:
- Standardized protocols for extracellular RNA isolation from cell culture supernatants are presented.
- The described methods offer a reliable and accessible approach for researchers.
- These protocols support the advancement of exRNA-based biomarker discovery and the understanding of intercellular communication.
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