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Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile
Published on: June 13, 2016
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Isolation of Extracellular RNA from Bile
Irene K Yan1, Valentin X Berdah2, Tushar Patel2
1Department of Transplantation, Mayo Clinic, Jacksonville, FL, USA. Yan.irene@mayo.edu.
Methods in Molecular Biology (Clifton, N.J.)
|February 2, 2018
Summary
Extracellular RNA from bile shows promise for biomarker discovery in biliary tract diseases and cancers. This study details optimized protocols for isolating this valuable RNA from bile samples.
Area of Science:
- Biochemistry
- Molecular Biology
- Medical Diagnostics
Background:
- Extracellular RNA (exRNA) is emerging as a significant tool for biomarker discovery.
- Extracellular vesicles in bodily fluids carry various RNA species, including mRNA and noncoding RNA.
- Bile is a readily accessible biofluid for potential disease marker analysis.
Purpose of the Study:
- To establish and optimize protocols for the isolation of extracellular RNA from bile.
- To facilitate the use of bile exRNA as a diagnostic tool for biliary tract diseases and cancers.
Main Methods:
- Modification of commercially available kits for RNA isolation.
- Detailed protocols for collection and processing of bile samples.
- Isolation of extracellular RNA from processed bile.
Main Results:
- Successfully adapted existing kits for bile exRNA isolation.
- Developed a reproducible method for collecting and processing bile for RNA analysis.
- Demonstrated the feasibility of obtaining extracellular RNA from bile.
Conclusions:
- Optimized protocols enable reliable isolation of extracellular RNA from bile.
- Bile exRNA holds potential as a non-invasive biomarker for biliary tract conditions.
- Further research can leverage these protocols for disease diagnostics and research.
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