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Updated: Feb 15, 2026

RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs
Published on: July 11, 2020
Detection and Analysis of Non-vesicular Extracellular RNA
Juan Pablo Tosar1,2, Alfonso Cayota3,4
1Functional Genomics Unit, Institut Pasteur de Montevideo, Montevideo, Uruguay.
Abstract:
Although extracellular vesicles (EVs) are by far the most studied carriers of extracellular small RNAs involved in cell-to-cell communication, most extracellular small RNAs are actually present as soluble vesicle-free supramolecular complexes. Several proteins have been described as the counterparts of these RNase-protected complexes. Here we describe a method for the purification and analysis of non-vesicular extracellular RNA derived from the conditioned media of mammalian cell culture. Focus on this fraction will increase our understanding on extracellular RNA biology, while serving as a source for biomarker discovery complementary to EVs.
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