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Evaluation of teicoplanin and vancomycin disk susceptibility tests
Journal of Clinical Microbiology
|January 1, 1986
Summary
This study recommends updated zone size standards for disk diffusion tests of teicoplanin and vancomycin antibiotics. These changes aim to improve the accuracy of susceptibility testing for glycopeptide antibiotics.
Area of Science:
- Microbiology
- Clinical Pharmacy
- Antibiotic Resistance
Background:
- Disk diffusion tests are crucial for determining antibiotic susceptibility.
- Glycopeptide antibiotics like vancomycin and teicoplanin are vital for treating resistant bacterial infections.
- Accurate susceptibility testing ensures effective antibiotic selection and management of infections.
Purpose of the Study:
- To evaluate disk diffusion test performance for teicoplanin and vancomycin.
- To compare these results with minimum inhibitory concentrations (MICs) of other antibiotics.
- To propose revised zone size interpretive standards for clinical laboratories.
Main Methods:
- Disk diffusion assays were performed using 30-microgram disks of vancomycin and teicoplanin.
- Results were compared against established MIC breakpoints.
- Interpretive zone sizes for susceptible and resistant categories were analyzed.
Main Results:
- Recommended new zone size standards for vancomycin: ≤10 mm (resistant), ≥15 mm (susceptible).
- Recommended new zone size standards for teicoplanin: ≤10 mm (resistant), ≥14 mm (susceptible).
- Currently, resistant clinical isolates are unavailable, making routine susceptibility testing infrequent.
Conclusions:
- Revised zone size standards enhance the reliability of vancomycin and teicoplanin disk diffusion tests.
- These updated standards aid in guiding appropriate glycopeptide antibiotic therapy.
- Further validation is needed as resistant strains become more prevalent.