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Electrically induced changes in amaranth seed enzymatic activity and their effect on bioactive compounds content
César Ozuna1, Abel Cerón-García1, Ma Elena Sosa-Morales1
1Departamento de Alimentos, División de Ciencias de la Vida, Campus Irapuato-Salamanca, Universidad de Guanajuato, Carretera Irapuato-Silao km 9, Ex Hacienda El Copal, C.P. 36500 Irapuato, Guanajuato Mexico.
Abstract:
Electric treatment applied to seeds and sprouts can change their phytochemical composition. However, only a handful of studies have investigated the effects of treating seeds with electric current prior to their germination on the enzymatic antioxidant system of their sprouts. The aim of this study was to determine the changes in bioactive compounds and the enzymatic antioxidant activities in seeds and amaranth sprouts under direct electric current (DC) treatments. Amaranth seeds were treated with DC at 500 mA for different periods of time (0, 2, 5, 10 and 30 min) and let sprout (85% RH, 25 ± 2 °C) for 6 days. Significant changes were found in the antioxidant enzymatic activities and in the total content of flavonoids (15.44 ± 0.56 mg RE/gDW) and phenolic compounds (35.87 ± 0.17 mg GAE/gDW) in 6-day-old sprouts from DC-treated seeds in comparison to sprouts form non-treated seeds. The results suggested that DC treatment for short period (5 min) can induce quantitative changes to the enzymatic antioxidant system of amaranth sprouts, thus representing a relatively cost-effective method for enhancing health-improving properties of sprouts.
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