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Lysobacter spongiae sp. nov., isolated from spongin
Heejae Choi1, Wan-Taek Im2, Jin-Sook Park3
1Department of Biological Sciences and Biotechnology, Hannam University, Daejeon, 34430, Republic of Korea.
Journal of Microbiology (Seoul, Korea)
|February 3, 2018
Summary
A novel bacterial strain, Lysobacter spongiae sp. nov., was isolated from spongin. This Gram-negative bacterium expands the known diversity within the Lysobacter genus, confirmed through comprehensive genomic and phenotypic analysis.
Area of Science:
- Microbiology
- Bacterial Taxonomy
- Marine Biology
Background:
- The genus Lysobacter encompasses Gram-negative, aerobic bacteria known for diverse metabolic capabilities.
- Spongin, a structural protein in sponges, can harbor unique microbial communities.
- Characterizing novel isolates is crucial for understanding microbial diversity and ecological roles.
Purpose of the Study:
- To taxonomically classify a novel bacterial strain isolated from spongin.
- To determine the phylogenetic placement of the isolate within the Lysobacter genus.
- To propose a new species name based on genotypic and phenotypic data.
Main Methods:
- Isolation and cultivation of bacterial strain 119BY6-57T on marine agar.
- Polyphasic taxonomic analysis including 16S rRNA gene sequencing.
- Genomic DNA G+C content determination, fatty acid analysis, and polar lipid profiling.
- DNA-DNA hybridization to assess relatedness with closely related species.
Main Results:
- Strain 119BY6-57T is a Gram-negative, motile, aerobic, rod-shaped bacterium.
- 16S rRNA gene sequence analysis indicated close relatedness to Lysobacter aestuarii (99.8% similarity).
- Genomic and phenotypic data, including low DNA-DNA relatedness (<48.0%), supported its distinction from known Lysobacter species.
- Major respiratory quinone was Q-8; major fatty acids included iso-C16:0, iso-C15:0, and summed features 3 and 9.
Conclusions:
- Strain 119BY6-57T represents a novel species within the genus Lysobacter.
- The proposed name for this new species is Lysobacter spongiae sp. nov.
- The type strain is 119BY6-57T (= KACC 19276T = LMG 30077T).
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