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Updated: Feb 15, 2026

Studying the Stoichiometry of Epidermal Growth Factor Receptor in Intact Cells using Correlative Microscopy
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Radiotherapy Upregulates Programmed Death Ligand-1 through the Pathways Downstream of Epidermal Growth Factor

Xing Song1, Yingjie Shao2, Tianwei Jiang1

  • 1Department of Tumor Biological Treatment, The Third Affiliated Hospital of Soochow University, Jiangsu, Changzhou 213003, China; Jiangsu Engineering Research Center for Tumor Immunotherapy, The Third Affiliated Hospital of Soochow University, Jiangsu, Changzhou 213003, China.

Ebiomedicine
|February 4, 2018
PubMed
Abstract

Insights

Radiotherapy up-regulates programmed death ligand-1 (PD-L1) in glioma by activating the epidermal growth factor receptor (EGFR) pathway. Inhibiting EGFR abrogates this effect, suggesting a therapeutic target for enhancing cancer immunotherapy.

Area of Science:

  • Oncology
  • Immunology
  • Molecular Biology

Background:

  • Radiotherapy (RT) can influence the tumor immune microenvironment.
  • Programmed death ligand-1 (PD-L1) is a key immune checkpoint molecule.
  • The epidermal growth factor receptor (EGFR) pathway is implicated in various cancers.

Purpose of the Study:

  • To investigate the role of the EGFR pathway in RT-induced PD-L1 up-regulation in glioma.
  • To explore the correlation between PD-L1 and EGFR expression in glioma specimens.

Main Methods:

  • Analysis of tissue microarrays from 64 glioma patients to correlate PD-L1 and EGFR levels.
  • In vitro experiments using U87 and U251 human glioma cell lines.
  • Assessment of PD-L1, phosphorylated EGFR, and JAK2 expression following RT and EGFR inhibition.

Main Results:

  • Significant correlation found between PD-L1 and EGFR expression in glioma (P=0.025).
  • RT significantly up-regulated PD-L1, phosphorylated EGFR, and JAK2 expression (P<0.05).
  • EGFR pathway inhibition abrogated RT-induced PD-L1 up-regulation, with comparable antitumor effects to PD-L1 blockade.

Conclusions:

  • Radiotherapy up-regulates PD-L1 expression in glioma via pathways downstream of EGFR.
  • Targeting the EGFR pathway presents a potential strategy to enhance RT efficacy in glioma treatment.
Keywords:
Combination therapyEGFRGliomaPD-L1Radiotherapy

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