Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Chromosome Replication02:31

Chromosome Replication

10.8K
Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin...
10.8K
Replicative Cell Senescence02:15

Replicative Cell Senescence

4.4K
Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds...
4.4K
DNA Replication02:40

DNA Replication

60.4K
DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied.  After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
DNA replication...
60.4K
Replication in Prokaryotes02:35

Replication in Prokaryotes

99.4K
Overview
99.4K
Replication in Prokaryotes01:32

Replication in Prokaryotes

28.2K
DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
28.2K
Replication in Eukaryotes02:31

Replication in Eukaryotes

205.9K
Overview
205.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Beta cell-targeted PD-1 agonist inhibits cell-mediated autoimmunity in pancreas tissue slices.

Science advances·2026
Same author

Identification of Early Symptoms Associated with Subsequent Immune-related Adverse Events in the I-SPY clinical trial.

Research square·2026
Same author

Harmine Selectively Drives Human Beta Cell Differentiation and Function Via Protein Kinase A Pathways.

bioRxiv : the preprint server for biology·2026
Same author

Corrigendum to "Evaluating antimicrobial efficacy in medical devices: The critical role of simulating in use test conditions" [Biomater. Adv. 172 (2025), 214241].

Biomaterials advances·2026
Same author

A Covalent Allosteric Molecular Glue Suppresses NRF2-Dependent Cancer Growth.

Cancer discovery·2025
Same author

TDP-43 directly inhibits mRNA accumulation in neurites through modulation of mRNA stability.

The EMBO journal·2025
Same journal

The BRCA1-A complex restricts replication fork reversal-dependent DNA repair in ATM deficient cells.

Nature communications·2026
Same journal

Signaling downstream of tumor-stroma interaction regulates mucinous colorectal adenocarcinoma apicobasal polarity.

Nature communications·2026
Same journal

Click-polymerized polyenamine membranes for efficient lithium extraction.

Nature communications·2026
Same journal

Joint trajectories of brain atrophy, white matter hyperintensities and cognition quantify brain maintenance.

Nature communications·2026
Same journal

Proton shuttling at electrochemical interfaces under alkaline hydrogen evolution.

Nature communications·2026
Same journal

metilene<sup>3</sup>: identifying DMRs across multiple conditions with auto-classification.

Nature communications·2026
See all related articles

Related Experiment Video

Updated: Feb 15, 2026

A High-content In Vitro Pancreatic Islet &#946;-cell Replication Discovery Platform
09:35

A High-content In Vitro Pancreatic Islet β-cell Replication Discovery Platform

Published on: July 16, 2016

11.7K

Replication confers β cell immaturity.

Sapna Puri1, Nilotpal Roy1, Holger A Russ1,2

  • 1Diabetes Center, Department of Medicine, University of California, San Francisco, CA, USA.

Nature Communications
|February 4, 2018
PubMed
Summary
This summary is machine-generated.

Pancreatic beta cells

More Related Videos

Generation of Immature, Mature and Tolerogenic Dendritic Cells with Differing Metabolic Phenotypes
06:09

Generation of Immature, Mature and Tolerogenic Dendritic Cells with Differing Metabolic Phenotypes

Published on: June 22, 2016

24.0K
Author Spotlight: Investigating Immune Cell Dynamics in the Tumor Microenvironment &#8212; Challenges and Innovations in Cancer Prognosis
07:32

Author Spotlight: Investigating Immune Cell Dynamics in the Tumor Microenvironment — Challenges and Innovations in Cancer Prognosis

Published on: April 12, 2024

2.0K

Related Experiment Videos

Last Updated: Feb 15, 2026

A High-content In Vitro Pancreatic Islet &#946;-cell Replication Discovery Platform
09:35

A High-content In Vitro Pancreatic Islet β-cell Replication Discovery Platform

Published on: July 16, 2016

11.7K
Generation of Immature, Mature and Tolerogenic Dendritic Cells with Differing Metabolic Phenotypes
06:09

Generation of Immature, Mature and Tolerogenic Dendritic Cells with Differing Metabolic Phenotypes

Published on: June 22, 2016

24.0K
Author Spotlight: Investigating Immune Cell Dynamics in the Tumor Microenvironment &#8212; Challenges and Innovations in Cancer Prognosis
07:32

Author Spotlight: Investigating Immune Cell Dynamics in the Tumor Microenvironment — Challenges and Innovations in Cancer Prognosis

Published on: April 12, 2024

2.0K

Area of Science:

  • Cell Biology
  • Endocrinology
  • Metabolism

Background:

  • Pancreatic beta cells regulate blood glucose via insulin secretion.
  • Adult beta cell replication is restricted, unlike robust neonatal proliferation.
  • Neonatal beta cells are functionally immature with lower glucose-stimulated insulin secretion.

Purpose of the Study:

  • To investigate the link between beta cell proliferation and functional immaturity.
  • To explore the role of c-Myc in regulating beta cell replication and phenotype.
  • To understand the balance between beta cell functionality and proliferative capacity.

Main Methods:

  • Induction of replication in adult beta cells.
  • Analysis of gene expression and metabolic profiles.
  • Assessment of glucose-stimulated insulin secretion.

Main Results:

  • Adult beta cells induced to replicate exhibit immature phenotypes.
  • c-Myc expression levels are tuned in proliferating beta cells.
  • Cell cycle entry promotes a functionally immature beta cell state.

Conclusions:

  • Beta cell proliferation and functional immaturity are interconnected.
  • A trade-off exists between mature beta cell function and proliferative potential.
  • Pro-proliferative cues induce a reversion to a less functional beta cell phenotype.