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MicroRNA-378 regulates cell proliferation and migration by repressing RNF31 in pituitary adenoma

Peng Qiu1, Tong-Jiang Xu2, Xiang-Dong Lu2

  • 1Department of Neurosurgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China.

Oncology Letters
|February 6, 2018
PubMed

Insights

MicroRNA-378 (miR-378) is downregulated in pituitary adenoma (PA) and inhibits tumor cell growth and migration. Restoring miR-378 or reducing RNF31 may offer new therapeutic strategies for PA.

Area of Science:

  • Endocrinology
  • Molecular Biology
  • Oncology

Background:

  • MicroRNA-378 (miR-378) is implicated in various cancers, but its role in pituitary adenoma (PA) is unclear.
  • Pituitary adenomas are tumors originating from the pituitary gland, and understanding their molecular drivers is crucial for effective treatment.

Purpose of the Study:

  • To investigate the expression and functional role of miR-378 in the development and progression of human pituitary adenoma.
  • To elucidate the molecular mechanism underlying miR-378's function in PA, focusing on its interaction with ring finger protein 31 (RNF31).

Main Methods:

  • Quantitative polymerase chain reaction (qPCR) to assess miR-378 and RNF31 expression in PA tissues and cells.
  • Cell proliferation assays (CCK-8) and cell migration assays (wound healing) to evaluate the functional impact of miR-378 and RNF31.
  • Transfection with miR-378 mimics and RNF31 knockdown in GH3 PA cells.

Main Results:

  • miR-378 expression was significantly downregulated in PA tissues compared to normal tissues.
  • Overexpression of miR-378 inhibited proliferation and migration of GH3 PA cells.
  • RNF31 was upregulated in PA and negatively regulated by miR-378; RNF31 knockdown suppressed PA cell proliferation and migration.

Conclusions:

  • The miR-378/RNF31 axis plays a critical role in the tumorigenesis and progression of human pituitary adenoma.
  • miR-378 acts as a tumor suppressor in PA by targeting RNF31.
  • This study identifies miR-378 and RNF31 as potential therapeutic targets for PA treatment.
Keywords:
miR-378migrationpituitary adenomasproliferationring finger protein 31

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