Related Experiment Video
Updated: Feb 15, 2026
08:58
Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques
Published on: July 5, 2018
13.2K
Characterizing bacterial glycoproteins with LC-MS.
Kelly M Fulton1, Jianjun Li1, Juan M Tomas2
1a Human Health Therapeutics Portfolio , National Research Council Canada , Ottawa , Canada.
Expert Review of Proteomics
|February 6, 2018
Summary
Bacterial glycoproteins are understudied virulence factors. Mass spectrometry advances enable their identification and characterization, revealing potential for new antimicrobials and vaccines.
Area of Science:
- Microbiology
- Glycobiology
- Analytical Chemistry
Background:
- Bacterial glycoproteins remain understudied in pathogenesis despite their discovery in the 1970s.
- Their role in bacterial virulence is not well understood.
- Mass spectrometry (MS) is a key technology for studying these molecules.
Purpose of the Study:
- To review current and emerging mass spectrometry (MS) approaches for bacterial glycoprotein identification and characterization.
- To highlight the importance of liquid chromatography (LC) in these analyses.
- To underscore the clinical potential of bacterial glycoproteins.
Main Methods:
- Utilizes complementary mass spectrometry (MS) techniques: intact protein analysis, top-down, and bottom-up approaches.
- Employs specialized liquid chromatography (LC) for separation.
- Integrates multiple analytical strategies for comprehensive characterization.
Main Results:
- Mass spectrometry (MS) offers high sensitivity and versatility for bacterial glycoprotein studies.
- Combined LC-MS approaches are crucial for identification and characterization.
- Bacterial glycoproteins possess unique structures with potential clinical applications.
Conclusions:
- Understanding bacterial glycoproteins is essential for harnessing their clinical utility.
- Their unique structures and synthesis pathways are promising targets for novel antimicrobials.
- Bacterial carbohydrate structures may serve as vaccine antigens or biomarkers.
Keywords:
Mass spectrometryantimicrobialsbacterial glycoproteinglycanglycopeptideglycoproteinhost-pathogen interactionsliquid chromatographyvaccinesMore Related Videos
Related Concept Videos
01:21Matrix Proteoglycans and Glycoproteins
5.2K
Proteoglycans are extensively glycosylated proteins, commonly found in the extracellular matrix, interwoven with collagen fibers. Hyaline cartilage, the most common type of cartilage in the body, consists of short and dispersed collagen fibers associated with large amounts of proteoglycans. These proteoglycans have long negative charges that attract cations, which in turn attract water molecules. This influx of ions and water molecules swells up the proteoglycan like a water-soaked gel that can...
5.2K
01:33Bacterial Transformation
60.2K
In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied two pneumonia strains in mice: one pathogenic and one non-pathogenic. Only the pathogenic strain killed host mice.
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
60.2K
01:30Bacterial Signaling
41.4K
Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...
41.4K
01:16Pharmacogenetics of Drug Transporters: P-Glycoprotein and Solute Carrier Transporters
4
The pharmacogenetics of drug transporters is increasingly recognized as a critical factor influencing interindividual variability in drug absorption, distribution, and elimination. These membrane-bound proteins regulate drugs' movement across cellular barriers by actively pumping them out (efflux) or facilitating their uptake (influx). Among the major transporter families, ATP-binding cassette (ABC) and solute carrier (SLC) transporters play particularly prominent roles. Genetic polymorphisms...
4
00:43Bacterial RNA Polymerase
32.9K
Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
32.9K
01:20Antiplatelet Drugs: Prostaglandin Synthesis, P2Y12 and Glycoprotein IIb/IIIa Inhibitors
1.3K
Antiplatelet drugs emerge as frontline defenders against the insidious threat of thromboembolic diseases, where abnormal clots obstruct vital blood vessels. These drugs stand as bulwarks, inhibiting platelet aggregation and clot formation, thereby mitigating the risk of life-threatening conditions like myocardial infarction, coronary artery disease, and thrombotic strokes.
Prostaglandin synthesis inhibitors, exemplified by the widely known aspirin, wield their power by irreversibly acetylating...
Prostaglandin synthesis inhibitors, exemplified by the widely known aspirin, wield their power by irreversibly acetylating...
1.3K