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Determination of phthalate esters in physiological saline solution by monolithic silica spin column extraction method
Lu Lu1,2, Yuki Hashi3, Zhi-Hua Wang1
1State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China.
Journal of Pharmaceutical Analysis
|February 7, 2018
Summary
A new eco-friendly method using monolithic silica spin column extraction (MonoSpin-SPE) and UFLC-MS accurately quantifies six phthalate esters in saline. Dimethyl- and diethyl-phthalate were detected in commercial saline samples.
Area of Science:
- Analytical Chemistry
- Environmental Science
Background:
- Phthalate esters are common plasticizers with potential health concerns.
- Accurate quantification of phthalates in medical solutions like physiological saline is crucial.
Purpose of the Study:
- To develop and validate a simple, sensitive, and eco-friendly method for determining six phthalate esters in physiological saline.
- To assess the presence of these phthalates in commercially available saline solutions.
Main Methods:
- Monolithic silica spin column extraction (MonoSpin-SPE) for sample pretreatment.
- Ultra-fast liquid chromatography-mass spectrometry (UFLC-MS) for separation and detection.
- Optimization of extraction and chromatographic conditions for linearity, sensitivity, and reproducibility.
Main Results:
- The method demonstrated good linearity (R² > 0.995) across specified ranges for six phthalate esters.
- Low limits of detection (0.02–0.9 μ/L) and quantification (0.08–2.7 μ/L) were achieved.
- High reproducibility (RSDs < 11.2%) and recovery rates (71.2%–107.3%) were obtained.
- Dimethyl-phthalate (DMP) and diethyl-phthalate (DEP) were detected in two brands of physiological saline.
Conclusions:
- MonoSpin-SPE combined with UFLC-MS is a suitable method for phthalate ester analysis in physiological saline.
- The presence of DMP and DEP in saline samples highlights the need for quality control in medical supplies.
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