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Formation of Dispersible Taohong Siwu Tablets
Published on: February 3, 2023
Development and validation of a normal-phase HPTLC method for the simultaneous analysis of Lamivudine and Zidovudine
Palani Venkatesh1, Mahesh Daggumati2
1Mother Theresa Post Graduate and Research Institute of Health Sciences, Pondicherry University, Pondicherry 605006, India.
Abstract:
Simultaneous quantification of Lamivudine and Zidovudine in tablets by HPTLC method was developed and validated. The chromatograms were developed using a mobile phase of toluene:ethyl acetate:methanol (4:4:2, v/v/v) on pre-coated plate of silica gel GF aluminum TLC plate and quantified by densitometric absorbance mode at 276 nm. The R values were 0.41±0.03 and 0.60±0.04 for Lamivudine and Zidovudine, respectively. The linearity of the method was found to be within the concentration range of 50-250 ng/spot for Lamivudine and for Zidovudine, it was 100-500 ng/spot. The lower limits of detection and quantification were 2.23 ng/spot and 7.90 ng/spot for Lamivudine and 2.90 ng/spot and 8.85 ng/spot for Zidovudine. The method was also validated for precision, specificity and recovery. This developed method was used to analyze fixed-dose tablets (Duovir, Cipla Ltd) samples of Lamivudine and Zidovudine.
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