Related Experiment Video
Updated: Feb 14, 2026

Use of Rabbit Eyes in Pharmacokinetic Studies of Intraocular Drugs
Published on: July 23, 2016
LC-MS/MS assay for the determination of natamycin in rabbit and human plasma: Application to a pharmacokinetics and
Yashpal Singh Chhonker1, Devendra Kumar1, Pankaj Shrivastava1
1CSIR-Central Drug Research Institute, Pharmacokinetics and Metabolism Division, Lucknow, UP 226001, India.
Abstract:
To enable reliable quantification of natamycin in rabbit and human plasma, a validated, sensitive and selective liquid chromatography-tandem mass spectrometry assay was developed. The chromatographic separation was achieved isocratically on a Cyano column using methanol: aqueous 3.5 mM ammonium acetate (pH 4) (90:10 v/v). The assay was validated over a concentration range of 6.25-400 ng/mL with lower limit of detection of 3.12 ng/mL. Quantification was performed using the transitions 664.5→137.2m/z for natamycin and 923.5→183.4m/z for the IS. The method was validated with respect to linearity, accuracy, precision, recovery and stability. This assay has been successfully applied to a pharmacokinetic study of natamycin in NZ rabbit and plasma protein binding in human plasma.
Related Concept Videos
Drug Distribution: Plasma Protein Binding
Physiological Pharmacokinetic Models: Assumption with Protein Binding
Nonlinear Pharmacokinetics: Bioavailability and Protein-Drug Binding
To quantify the extent of bioavailability, pharmacologists often use a parameter called .
Protein-Drug Binding: Determination Methods
Indirect methods involve isolating the bound drug from its free form in biological samples such as blood, serum, or plasma. These techniques aim to measure the percentage of drugs bound to proteins. Equilibrium dialysis is a commonly used method where the free drug concentration at equilibrium is measured by separating the bound...
Factors Affecting Protein-Drug Binding: Protein-Related Factors
The physicochemical properties of a drug play a significant role in its ability to bind to proteins. Lipophilic drugs, which dissolve in fats, oils, and lipids, can be...
The Equilibrium Binding Constant and Binding Strength

