Related Experiment Video
Updated: Feb 14, 2026

Immunoglobulin G N-Glycan Analysis by Ultra-Performance Liquid Chromatography
Published on: January 18, 2020
Fused-core particle technology in high-performance liquid chromatography: An overview
Joseph J Kirkland1, Stephanie A Schuster1, William L Johnson1
1Advanced Materials Technology, Inc., 3521 Silverside Road, Suite 1-K, Quillen Building, Wilmington, DE 19810, USA.
Superficially porous particles (SPPs) enhance high-performance liquid chromatography (HPLC) separations. This technology offers high efficiency and broad applicability across scientific disciplines.
Area of Science:
- Analytical Chemistry
- Separation Science
- Chromatography
Background:
- Superficially porous particles (SPPs) have revolutionized packed high-performance liquid chromatography (HPLC) column technology.
- SPPs offer high efficiency, convenient operating conditions, and compatibility with conventional HPLC instruments.
Purpose of the Study:
- To provide an overview of superficially porous particle (SPP) technology.
- To discuss the expansion of SPP products with varied physical parameters.
- To highlight the optimization of SPP for specific compound separations based on solute size.
Main Methods:
- Review of SPP technology development since 2006.
- Analysis of SPP product family characteristics (particle size, pore size).
- Examination of application-specific optimization strategies for SPP columns.
Main Results:
- SPP technology provides significantly improved chromatographic efficiency.
- A diverse range of SPP products are available, tailored for different separation needs.
- Optimization based on solute size enables targeted compound separation.
Conclusions:
- SPP technology represents a significant advancement in HPLC.
- The versatility of SPP columns supports widespread applications in various scientific fields.
- Tailored SPP designs enhance separation performance for specific compound classes.
More Related Videos
10:52Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation
Published on: January 6, 2016
06:25Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
Published on: April 26, 2016
Related Concept Videos
The Nucleosome Core Particle
The paradox
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their main responsibility is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. While on the other hand, they must allow polymerase enzymes to access DNA...
The Nucleosome Core Particle
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their primary aim is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. On the other hand, they must allow polymerase enzymes to access histone-bound DNA during...
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
High-Performance Liquid Chromatography: Instrumentation
High-Performance Liquid Chromatography: Elution Process
High-Performance Liquid Chromatography: Types of Detectors