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Nanoliter Sensing for Infrared Bioanalytics.

Christoph Kratz¹, Andreas Furchner¹, Thomas W H Oates¹

¹Leibniz-Institut für Analytische Wissenschaften - ISAS - e.V. , Schwarzschildstr. 8, 12489 Berlin, Germany.

|February 7, 2018

Summary

This summary is machine-generated.

This study introduces an optofluidic platform for sensitive, label-free bioanalysis of ultrathin biomolecular films. The novel approach achieves submonolayer sensitivity for detecting low analyte concentrations in small sample volumes.

Keywords:

absorption kinetic in situ IR spectroscopy microfluidics molecular sensing surface enhanced infrared absorption vibrational sensing

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Area of Science:

Optofluidics
Surface-enhanced infrared spectroscopy (SEIR)
Bioanalytical chemistry

Background:

Nondestructive, label-free bioanalysis of small sample volumes with low analyte concentrations demands innovative analytical methods.
Existing techniques may struggle with sensitivity and sample handling for ultrathin biomolecular films.

Purpose of the Study:

To develop and demonstrate an optofluidic platform integrating surface-enhanced in situ infrared spectroscopy (SEIR) with microfluidics.
To achieve sensitive detection of surface-immobilized ultrathin biomolecular films in liquid analytes.

Main Methods:

Utilized an optofluidic platform combining SEIR spectroscopy and microfluidics.
Employed nonfunctionalized and functionalized metal island films on silicon substrates for signal enhancement.
Implemented a single-reflection geometry below the attenuated-total-reflection (ATR) regime using IR-transparent windows to avoid strong IR absorption by aqueous environments and polymers.

Main Results:

Demonstrated submonolayer sensitivity down to a few ng/cm² for biomolecular adsorption.
Successfully detected the adsorption of glutathione and the recognition of streptavidin on a biotinylated substrate.
Showcased quantifiable signal enhancement properties of the metal island films.

Conclusions:

The developed optofluidic platform enables sensitive, label-free quantification of biomolecular interactions.
The method circumvents limitations associated with IR absorption in aqueous environments, allowing for straightforward analysis of adsorption kinetics and molecular properties.