Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Endogenous near-infrared chemiluminescent manganese ion-driven porphyrin supramolecular platform for the theranostics of thrombosis and ischemic stroke.

RSC advances·2026
Same author

Millimeter-Wave MIMO radar for contactless neonatal heart-rate assessment: performance under common resuscitation-related maneuvers and feasibility in clinical workflow.

Frontiers in pediatrics·2026
Same author

Supramolecular Organic Framework-Enabled AIE-Enhanced Electrochemiluminescence: Dual-Readout Ultrasensitive Determination of Microcystin-LR in Environmental Waters.

Analytical chemistry·2026
Same author

Electron-Ion Coupling for Nanostructured Photocathode in Ion-Assisted Photoelectrochemical Microfluidic Biosensing Platform.

Analytical chemistry·2026
Same author

Epidemiological trends and geographic disparities in low back pain burden based on the 2021 GBD study: A cross-sectional analysis.

Medicine·2026
Same author

Inhibition of the <i>in vitro</i> colonic fermentation of cooked gluten by dietary fibers with individual fermentability.

Food & function·2026

Related Experiment Video

Updated: Jul 7, 2026

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

Fluidic Lipid-Bilayer-Enhanced Iontronic Nanopore: Machine-Learning-Driven Ultrasensitive MicroRNA Detection in

Yanlei Li1, Saimei Zhang2, Xiang Ren1

  • 1Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, P. R. China.

ACS Sensors
|July 6, 2026
PubMed
Summary

We developed a dynamic DNA walker on a fluidic supported lipid bilayer for ultrasensitive microRNA detection. This novel approach enhances reaction kinetics, enabling rapid and accurate diagnostics for precision medicine.

Keywords:
DNA walkeriontronic sensingmachine learning diagnosticsmicroRNA detectionnanopore biosensorsupported lipid bilayer

More Related Videos

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood
08:58

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood

Published on: April 16, 2016

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
07:27

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

Related Experiment Videos

Last Updated: Jul 7, 2026

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood
08:58

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood

Published on: April 16, 2016

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
07:27

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

Area of Science:

  • Biotechnology
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Ultrasensitive microRNA (miRNA) detection is vital for precision diagnostics.
  • Conventional surface-based DNA walkers face limitations due to slow kinetics on static substrates.

Purpose of the Study:

  • To develop a dynamic DNA walker system for enhanced miRNA detection.
  • To overcome the kinetic limitations of traditional DNA walkers using a fluidic substrate.

Main Methods:

  • Utilized a fluidic supported lipid bilayer (SLB) integrated with an anodic aluminum oxide (AAO) nanopore.
  • Employed fluorescence recovery after photobleaching (FRAP) to confirm probe mobility.
  • Investigated signal transduction via pore diameter and surface charge density, triggering a conductance switch.

Main Results:

  • Demonstrated lateral mobility of lipid-anchored probes with a diffusion coefficient of ≈ 1.2 μm²/s.
  • Shifted the system to a reaction-controlled regime (Damköhler number <1), achieving equilibrium in 30 min.
  • Achieved a limit of detection of 25 aM for microRNA-21 (miR-21) with >98% accuracy in clinical sample classification.

Conclusions:

  • Established substrate fluidity as a key design principle for next-generation iontronic sensors.
  • The dynamic DNA walker platform offers rapid, ultrasensitive, and accurate miRNA detection.
  • This technology holds significant promise for advancing precision diagnostics and clinical sample analysis.