Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cell Migration01:19

Cell Migration

6.7K
Cell migration is a process by which the cells move from one location to another, playing an essential role in embryological development, repair and regeneration, immune response, and metastasis. Cells migrate in response to chemical or mechanical signals generated by specific organs or tissues. The overall mechanism includes three steps - polarization, protrusion, and release. Polarization involves the formation of a distinct cell front and rear, which determines the direction of movement.
6.7K
Cell Migration01:09

Cell Migration

18.9K
Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.
18.9K
Cancer Cell Migration through Invadopodia01:35

Cancer Cell Migration through Invadopodia

3.3K
Invadosome is a broad category of cell surface structures with proteolytic activity that  degrades the extracellular matrix (ECM). Invadosomes are present in normal cell types, including macrophages, endothelial cells, and neurons, as well as tumor cells. Although the macrophage podosomes and tumor cell invadopodia are classified as invadosomes, they have different structures, molecular pathways, and functions. Podosomes are short structures that last for a few minutes. However,...
3.3K
Chemotaxis and Direction of Cell Migration01:21

Chemotaxis and Direction of Cell Migration

5.8K
Cells can detect chemical cues in their environment and reorganize the cytoskeleton to migrate toward them or away from them. This directional migration, called chemotaxis, is essential during embryogenesis and development, immune response, tissue repair and regeneration, and reproduction. These chemical cues can either attract or repel the cell's movement. For example, axon development is determined by a combination of chemoattractants and chemorepellents that direct the growing axon...
5.8K
Role of Myosin in Cell Migration01:18

Role of Myosin in Cell Migration

3.4K
Myosins are multimeric motor proteins involved in various cellular processes such as migration, adhesion, and proliferation. Myosin II is the most common type in animal cells, which binds and cross-links actin filaments.
Myosin II  is a hexamer comprising two heavy chains with globular heads and coiled-coil tails, two regulatory light chains, and two essential light chains. The ATPase sites on the myosin heads hydrolyze ATP, and the released phosphate generates the force for contraction....
3.4K
Cytoskeletal Coordination in Cell Migration01:32

Cytoskeletal Coordination in Cell Migration

5.6K
A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
5.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Live human metapneumovirus vaccine candidates attenuated by temperature sensitivity mutations from human respiratory syncytial virus.

Journal of virology·2026
Same author

Toward molecular and pathology-confirmed completeness in advanced ovarian cancer cytoreduction: Intraoperative molecular imaging and integrated theranostic strategies.

Molecular aspects of medicine·2026
Same author

In silico design of CLDN4-directed peptide-loaded nanobubbles for ultrasound-assisted delivery, photothermal therapy, immune activation, and multimodal imaging in ovarian cancer.

Journal of nanobiotechnology·2026
Same author

Generic optimization procedure for high-resolution printing by stereolithography of 3D scaffolds compatible for cell culture applied to a small intestinal architecture.

Biofabrication·2026
Same author

Condensates in fusion oncoprotein-driven leukemia: new biology and therapeutic opportunities.

Trends in cancer·2026
Same author

The Role of Phase-Separated Condensates in Fusion Oncoprotein-Driven Cancers.

Annual review of cancer biology·2026

Related Experiment Video

Updated: Feb 14, 2026

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
11:32

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen

Published on: May 24, 2017

12.7K

Mapping Interactions between p27 and RhoA that Stimulate Cell Migration.

Aaron H Phillips1, Li Ou1, Alexandre Gay2

  • 1Department of Structural Biology, St. Jude Children's Research Hospital, 262 Danny Thomas Place, Memphis, TN 38105, United States.

Journal of Molecular Biology
|February 8, 2018
PubMed
Summary
This summary is machine-generated.

The protein p27, when in the cytoplasm, can promote cancer cell migration by interacting with RhoA. This interaction, though weak, requires the full p27 protein, suggesting other factors may be involved.

Keywords:
IDPsRhocell migrationp27(Kip1)protein interactions

More Related Videos

Co-culture of Glioblastoma Stem-like Cells on Patterned Neurons to Study Migration and Cellular Interactions
10:08

Co-culture of Glioblastoma Stem-like Cells on Patterned Neurons to Study Migration and Cellular Interactions

Published on: February 24, 2021

6.9K
Real-time Imaging of Leukotriene B4 Mediated Cell Migration and BLT1 Interactions with β-arrestin
13:45

Real-time Imaging of Leukotriene B4 Mediated Cell Migration and BLT1 Interactions with β-arrestin

Published on: December 23, 2010

13.3K

Related Experiment Videos

Last Updated: Feb 14, 2026

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
11:32

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen

Published on: May 24, 2017

12.7K
Co-culture of Glioblastoma Stem-like Cells on Patterned Neurons to Study Migration and Cellular Interactions
10:08

Co-culture of Glioblastoma Stem-like Cells on Patterned Neurons to Study Migration and Cellular Interactions

Published on: February 24, 2021

6.9K
Real-time Imaging of Leukotriene B4 Mediated Cell Migration and BLT1 Interactions with β-arrestin
13:45

Real-time Imaging of Leukotriene B4 Mediated Cell Migration and BLT1 Interactions with β-arrestin

Published on: December 23, 2010

13.3K

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biophysics

Background:

  • p27 normally inhibits cell cycle progression by binding cyclin-dependent kinases.
  • Mislocalized cytoplasmic p27 can promote pro-oncogenic signaling, including cell migration.
  • Cytoplasmic p27 interacts with RhoA, hindering guanine nucleotide exchange and promoting migration.

Purpose of the Study:

  • To investigate the biophysical interaction between p27 and RhoA.
  • To determine the role of p27 domains in RhoA interaction and cell migration.
  • To elucidate the mechanism by which cytoplasmic p27 promotes cell migration.

Main Methods:

  • In vitro biophysical assays to measure p27-RhoA binding affinity.
  • Site-directed mutagenesis to assess the role of p27 domains.
  • Scratch wound healing assays to evaluate cell migration.
  • Guanine nucleotide exchange assays for RhoA.

Main Results:

  • Direct interaction between the N-terminus of p27 and RhoA was confirmed in vitro.
  • The affinity of p27 for RhoA is low (hundreds of micromolar dissociation constant).
  • High concentrations of p27 inhibit guanine nucleotide exchange factor-mediated nucleotide exchange from RhoA.
  • Full-length p27 is required for promoting cell migration, while the C-terminus is dispensable for direct in vitro interaction with RhoA.

Conclusions:

  • Cytoplasmic p27 directly interacts with RhoA, contributing to cell migration.
  • The interaction's low affinity suggests a potential role for additional factors.
  • The C-terminus of p27 may associate with unknown factors to enhance RhoA binding and promote migration.