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Redox Capacitive Assaying of C-Reactive Protein at a Peptide Supported Aptamer Interface
Julia Piccoli1, Robert Hein2, Afaf H El-Sagheer2,3
1Institute of Chemistry , São Paulo State University (UNESP) , 14800-900 , Araraquara , São Paulo , Brazil.
Abstract:
Electrochemical immunosensors offer much in the potential translation of a lab based sensing capability to a useful "real world" platform. In previous work we have introduced an impedance-derived electrochemical capacitance spectroscopic analysis as supportive of a reagentless means of reporting on analyte target capture at suitably prepared mixed-component redox-active, antibody-modified interfaces. Herein we directly integrate receptive aptamers into a redox charging peptide support in enabling a label-free low picomolar analytical assay for C-reactive protein with a sensitivity that significantly exceeds that attainable with an analogous antibody interface.
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