Related Experiment Video
Updated: Feb 14, 2026

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
Published on: April 25, 2025
Heterogeneous asymmetric recombinase polymerase amplification (haRPA) for rapid hygiene control of large-volume water
Dennis Elsäßer1, Johannes Ho2, Reinhard Niessner1
1Institute of Hydrochemistry, Chair of Analytical Chemistry and Water Chemistry, Technical University of Munich, Marchioninistrasse 17, 81377, München, Germany.
Abstract:
Hygiene of drinking water is periodically controlled by cultivation and enumeration of indicator bacteria. Rapid and comprehensive measurements of emerging pathogens are of increasing interest to improve drinking water safety. In this study, the feasibility to detect bacteriophage PhiX174 as a potential indicator for virus contamination in large volumes of water is demonstrated. Three consecutive concentration methods (continuous ultrafiltration, monolithic adsorption filtration, and centrifugal ultrafiltration) were combined to concentrate phages stepwise from 1250 L drinking water into 1 mL. Heterogeneous asymmetric recombinase polymerase amplification (haRPA) is applied as rapid detection method. Field measurements were conducted to test the developed system for hygiene online monitoring under realistic conditions. We could show that this system allows the detection of artificial contaminations of bacteriophage PhiX174 in drinking water pipelines.
More Related Videos
08:37Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
Published on: March 30, 2015
09:03Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a
Published on: December 23, 2022
Related Concept Videos
RACE - Rapid Amplification of cDNA Ends
Control Volume and System Representations
The control volume approach considers a stationary region in space through which fluid flows. This region is bounded by a control surface. For instance, in the case of water...
Linear Momentum in Control Volume
Conservation of Energy in Control Volume
For steady flow systems, the time derivative of the stored energy becomes zero since there is no energy accumulation within the control volume. This simplifies the energy equation to:
Hand hygiene
Hand washing...
Translesion DNA Polymerases
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...