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Updated: Feb 14, 2026

Extraction of Aqueous Metabolites from Cultured Adherent Cells for Metabolomic Analysis by Capillary Electrophoresis-Mass Spectrometry
Published on: June 9, 2019
Metabolite-Induced Protein Expression Guided by Metabolomics and Systems Biology
Martin Giera1, Filipe Branco Dos Santos2, Gary Siuzdak3
1Leiden University Medical Center, Center for Proteomics and Metabolomics, Albinusdreef 2, 2300RC Leiden, the Netherlands.
Abstract:
Metabolomics combined with systems biology can be used to identify endogenous metabolites that modulate protein expression. Recent examples include the 2-fold enhancements of pertussis toxin protein in vaccine production and myelin basic protein expression in oligodendrocyte maturation; both applied a metabolomics-systems strategy to identify active metabolites.
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