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Related Concept Videos

CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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Immunological memory, a pivotal pillar of the adaptive immune system, is responsible for the body's ability to remember and respond more swiftly and effectively to previously encountered pathogens. This remarkable feature is what makes vaccines so effective in preventing diseases.
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The Antiviral System of Bacteria and Archaea: CRISPR01:23

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CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
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Microsoft Excel is a cornerstone tool for data analysis and statistical operations, offering a wide array of functionalities to manage, analyze, and visualize data efficiently. Recognized for its versatility, Excel facilitates the performance of basic to complex statistical operations, serving as an indispensable asset for analysts, researchers, and students alike. Excel's significance in data analysis emanates from its spreadsheet environment, where data can be organized in rows and...
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Using CRISPR/Cas9 to Knock Out GM-CSF in CAR-T Cells
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CRISPR/Cas9: A tool for immunological research.

Katharina Hochheiser1, Andrew J Kueh2,3, Thomas Gebhardt1

  • 1Department of Microbiology & Immunology, The University of Melbourne, the Peter Doherty Institute for Infection & Immunity, Melbourne, Victoria, Australia.

European Journal of Immunology
|February 8, 2018
PubMed
Summary
This summary is machine-generated.

The CRISPR/Cas9 system offers efficient genetic engineering for biomedical research, including in immunology and potential therapeutic applications. This powerful tool facilitates cell modification, disease modeling, and gene screening.

Keywords:
Animal modelsCRISPR/Cas9Clinical trialsGene therapy

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Immunology

Background:

  • CRISPR/Cas9 originated from bacterial adaptive immunity.
  • It has been repurposed for efficient genetic manipulation in eukaryotic systems.
  • This technology holds significant promise for future clinical therapeutics.

Purpose of the Study:

  • Discuss CRISPR/Cas9 applications in modifying hematopoietic cells.
  • Explain its use in generating mouse models for immunological research.
  • Highlight its utility as a screening tool for identifying genes in immune processes.

Main Methods:

  • Genetic modification of cell lines, primary cells, and organisms.
  • Application of CRISPR/Cas9 for generating disease models.
  • Utilizing CRISPR/Cas9 as a screening platform.

Main Results:

  • Demonstrated ease and efficiency in genetic manipulation.
  • Enabled the generation of specific mouse models for immunology.
  • Facilitated gene identification in immunological pathways.

Conclusions:

  • CRISPR/Cas9 is a versatile tool for biomedical and immunological research.
  • Its potential extends to therapeutic applications and advanced screening.
  • Ongoing developments include transcriptional activation and repression functionalities.