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Taurine reduces blue light-induced retinal neuronal cell apoptosis in vitro

Wu Dayang¹, Pang Dongbo¹

¹a Department of Ophthalmology , The First Affiliated Hospital of Jinzhou Medical University , Jinzhou , PR China.

Cutaneous and Ocular Toxicology

|February 9, 2018

Abstract

Background:

The massive uptake of organic compatible osmolytes is a self-protective response to multiple stressors.

Objective:

This study aimed to determine the protective effects of the osmolyte taurine against blue light-induced apoptosis in retinal neuronal cells in vitro.

Methods:

Real-time PCR was used to measure osmolyte transport. Radioimmunoassays were performed to measure osmolyte uptake. Cell Counting Kit-8 assays were conducted to measure cellular viability. Flow cytometry analysis was used to measure apoptosis.

Results:

Compared with normotonic stress, hypertonic stress-induced uptake of osmolytes, including betaine, myoinositol, and taurine, into the retinal neuronal cells. Blue light increased osmolyte transporter mRNA expression together with osmolyte uptake. Furthermore, taurine significantly suppressed blue light-induced retinal neuronal cell apoptosis.

Conclusion:

The compatible osmolyte taurine may have an important role in cell resistance to blue light and cell survival.

Keywords:

Blue light apoptosis osmolyte retina taurine

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