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Updated: Feb 14, 2026

Evaluating Leaf Responses to Microbial Secondary Metabolites Using A High-Throughput Format
Published on: December 5, 2025
Recent advances in reconstructing microbial secondary metabolites biosynthesis in Aspergillus spp
Yi He1, Bin Wang2, Wanping Chen3
1College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, Hubei Province 430023, PR China; Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, Wuhan, Hubei Province 430023, PR China; Hubei Key Laboratory for Processing and Transformation of Agricultural Products (Wuhan Polytechnic University), Wuhan, Hubei Province 430023, PR China.
Abstract:
High throughput genome sequencing has revealed a multitude of potential secondary metabolites biosynthetic pathways that remain cryptic. Pathway reconstruction coupled with genetic engineering via heterologous expression enables discovery of novel compounds, elucidation of biosynthetic pathways, and optimization of product yields. Apart from Escherichia coli and yeast, fungi, especially Aspergillus spp., are well known and efficient heterologous hosts. This review summarizes recent advances in heterologous expression of microbial secondary metabolite biosynthesis in Aspergillus spp. We also discuss the technological challenges and successes in regard to heterologous host selection and DNA assembly behind the reconstruction of microbial secondary metabolite biosynthesis.
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