Cross-linked hyaluronan films loaded with acetazolamide-cyclodextrin-triethanolamine complexes for glaucoma treatment
Javier Adrián Calles1,2,3, Maria Julia Mora4, Renée Onnainty4
1Institute of Applied Ophthalmo-Biology (IOBA), University of Valladolid, 47011, Valladolid, Spain.
Aim:
This work aimed to design and characterize cross-linked hyaluronic acid-itaconic acid films loaded with acetazolamide-hydroxypropyl β cyclodextrin-triethanolamine complexes.
Materials & Methods:
Films were cross-linked with itaconic acid and poly(ethyleneglycol)-diglycidylether. Biopharmaceutical properties were assessed by evaluating in vitro drug release rate, biocompatibility in a human corneal epithelial cell line, bioadhesiveness with pig gastric mucin, in vivo bioadhesion and efficacy.
Results:
Showed good mechanical properties and oxygen permeability. Proliferation rate of corneal cells was affected by highest acetazolamide concentration. Bioadhesive interaction exhibited a water movement from pig mucin to the film; in vivo experiments showed strong bioadhesion for 8 h and hypotensive effect for almost 20 h.
Conclusion:
Experimental set showed promising performance and encouraged future studies to optimize formulation. [Formula: see text].
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