Related Experiment Video
Updated: Feb 14, 2026

Fabrication of a Bioactive, PCL-based "Self-fitting" Shape Memory Polymer Scaffold
Published on: October 23, 2015
A disposable immunosensor using ITO based electrode modified by a star-shaped polymer for analysis of tumor
Muhammet Aydın1, Elif Burcu Aydın1, Mustafa Kemal Sezgintürk2
1Namık Kemal University, Scientific and Technological Research Center, Tekirdağ, Turkey.
Abstract:
Label-free immunosensor based on tetra armed star-shaped poly(glycidylmethacrylate) (StarPGMA) modified disposable ITO electrode was developed for detection of p53 protein, an important colorectal cancer biomarker. This disposable biosensor was fabricated by spin-coating technique using star-shaped StarPGMA with epoxy side groups. After formation of a stable film with epoxy ends, anti-p53 antibodies were bound to these groups covalently. Stepwise modification of the electrode was followed by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) studies. The electrochemical performance of the immunosensor was studied by EIS. Furthermore, the antibody and antigen coupling was monitored by single frequency impedance (SFI) technique. The immunosensor showed a low limit of detection (7 fg/mL) and a linear detection range between 0.02 pg/mL and 4 pg/mL. Additionally, atomic force microscopy (AFM) and scanning electron microscopy (SEM) were utilized for monitoring of immunosensor surface at different stages of fabrication. The antibody coupling on the electrode surface was proved by Fourier-transform infrared spectroscopy (FTIR) and Raman spectroscopy. Furthermore, the proposed immunosensor had good reproducibility and repeatability.
Related Concept Videos
Loss of Tumor Suppressor Gene Functions
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
Covalently Linked Protein Regulators
These groups modify specific amino acids in a protein....
Polymers
Cancer-Critical Genes II: Tumor Suppressor Genes
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
Molecular Shape and Polarity
Standard Electrode Potentials

