Related Experiment Video
Updated: Feb 14, 2026
03:45
Author Spotlight: Advancing Tissue Regeneration and Disease Modeling with Dental Pulp Stem Cells
Published on: May 5, 2023
3.3K
Exosomes with Highly Angiogenic Potential for Possible Use in Pulp Regeneration
Xuehong Xian1, Qimei Gong1, Chen Li1
1Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Affiliated Stomatological Hospital, Sun Yat-sen University, Guangzhou, People's Republic of China.
Journal of Endodontics
|February 11, 2018
Summary
Dental pulp cell-derived exosomes (DPC-Exos) promote blood vessel formation in human umbilical vein endothelial cells (HUVECs). Inhibiting p38 MAPK signaling further enhances this DPC-Exos-driven angiogenesis, crucial for pulp regeneration.
Area of Science:
- Cell Biology
- Biomedical Engineering
- Regenerative Medicine
Background:
- Angiogenesis is essential for dental pulp regeneration.
- Exosomes mediate paracrine signaling and modulate angiogenesis.
- The role of dental pulp cell-derived exosomes (DPC-Exos) in angiogenesis is largely unexplored.
Purpose of the Study:
- To investigate the proangiogenic properties of DPC-Exos.
- To determine the effect of DPC-Exos on human umbilical vein endothelial cells (HUVECs).
Main Methods:
- DPC-Exos were isolated using ultracentrifugation.
- Exosomes were characterized via transmission electron microscopy, Western blotting, and nanoparticle tracking analysis.
- In vitro assays assessed HUVEC proliferation, proangiogenic factor expression, and tube formation.
Main Results:
- Characterized DPC-Exos were successfully isolated.
- DPC-Exos significantly promoted HUVEC proliferation and tube formation.
- DPC-Exos increased the expression of proangiogenic factors.
- Inhibition of p38 mitogen-activated protein kinase (MAPK) signaling enhanced DPC-Exos-induced tube formation.
Conclusions:
- DPC-Exos play a significant role in promoting angiogenesis.
- p38 MAPK signaling inhibition potentiates the proangiogenic effects of DPC-Exos.
- These findings highlight the potential of DPC-Exos in regenerative therapies for dental pulp.
More Related Videos
08:19Transfer of Manipulated Tumor-associated Neutrophils into Tumor-Bearing Mice to Study their Angiogenic Potential In Vivo
Published on: July 20, 2019
6.5K
10:04The Aortic Ring Co-culture Assay: A Convenient Tool to Assess the Angiogenic Potential of Mesenchymal Stromal Cells In Vitro
Published on: September 18, 2017
11.7K
Related Concept Videos
01:36Overview of Exosomes
3.8K
Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
3.8K
00:52Potential Energy
43.0K
The energy stored by a structure and location of matter in space is called potential energy. For instance, raising a kettlebell changes its spatial location and increases its potential energy. Similarly, a stretched rubber band contains potential energy which, under certain conditions, can be converted into other forms of energy, such as kinetic energy.
Chemical bonds that form attractive forces between atoms also contain potential energy, called chemical energy. When a chemical reaction...
Chemical bonds that form attractive forces between atoms also contain potential energy, called chemical energy. When a chemical reaction...
43.0K
01:09Potential Energy
1.0K
A conservative force, such as a gravitational or elastic force, gives the body the capacity to do work. This capacity, measured as the potential energy, depends on the body's location or “position” relative to a fixed reference position or datum. The gravitational potential energy is considered zero at the reference point. Suppose a body is located at some vertical distance above a fixed horizontal reference or datum. In that case, the weight of the body has positive gravitational potential...
1.0K
01:33Whole Body Regeneration
4.2K
Regeneration is the process of restoring injured or lost tissues, organs, or body parts. While simpler organisms generally show greater ability to regenerate their whole body, few complex animals show similarly exceptional regeneration. For example, planarian flatworms have a unique regenerative potential making them a popular study organism among biologists to understand the mechanisms of whole body regeneration. Other organisms, such as hydra, also show extreme regeneration potential;...
4.2K
01:24Liver Regeneration
4.5K
The liver is an important organ in vertebrates that plays an essential role in metabolism. It is also responsible for storing and redistributing nutrients such as carbohydrates, fats, and vitamins in the body. Additionally, the liver releases bile salts which are critical for digesting food and eliminating toxic metabolites from the body.
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are...
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are...
4.5K
03:02Standard Electrode Potentials
50.6K
On comparing the reactivity of silver and lead, it is observed that the two ionic species, Ag+ (aq) and Pb2+ (aq), show a difference in their redox reactivity towards copper: the silver ion undergoes spontaneous reduction, while the lead ion does not. This relative redox activity can be easily quantified in electrochemical cells by a property called cell potential. This property is commonly known as cell voltage in electrochemistry, and it is a measure of the energy which accompanies the charge...
50.6K