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Updated: Feb 14, 2026

Preparation of Stable Bicyclic Aziridinium Ions and Their Ring-Opening for the Synthesis of Azaheterocycles
Published on: August 22, 2018
Synthesis of a Stable Analog of the Phosphorylated Form of CheY: Phosphono-CheY
Daniel B Lookadoo1, Matthew S Beyersdorf1, Christopher J Halkides2
1Department of Chemistry and Biochemistry, The University of North Carolina Wilmington, Wilmington, NC, USA.
Abstract:
CheY is a response regulator of bacterial chemotaxis that is activated by phosphorylation of a conserved aspartate residue. However, studies of CheY-phosphate have proven challenging due to rapid hydrolysis of the aspartyl-phosphate in vitro. To combat this issue, we have designed a stable analog suitable for structural and functional studies. Herein, we describe a method for the chemical modification of Thermotoga maritima CheY to produce a phospho-analog designated as phosphono-CheY. Our modification produces a stable analog in the constitutively active form that enables the study of signal transfer to the downstream target.
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