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Rapid Identification of Gram Negative Bacteria from Blood Culture Broth Using MALDI-TOF Mass Spectrometry
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Rapid Identification of Gram Negative Bacteria from Blood Culture Broth Using MALDI-TOF Mass Spectrometry

Published on: May 28, 2014

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Infection with gram-negative bacteria among children in a tertiary pediatric hospital in Egypt

John Rene Labib1, Sally K Ibrahim1, Marwa Rashad Salem2

  • 1Department of Pediatrics, Faculty of Medicine, Cairo University, Egypt.

American Journal of Infection Control
|February 13, 2018
PubMed

Insights

Pediatric intensive care units (PICUs) face high rates of gram-negative bacteria (GNB) infections, with a significant mortality rate. Multidrug-resistant (MDR) GNB infections lead to longer hospital stays, underscoring the need for surveillance.

Area of Science:

  • Pediatric Intensive Care
  • Infectious Diseases
  • Microbiology

Background:

  • Patients in pediatric intensive care units (PICUs) are at high risk for gram-negative bacteria (GNB) infections.
  • Gram-negative bacteria pose a significant threat to critically ill children.

Purpose of the Study:

  • To determine the incidence and outcomes of GNB infections in a PICU.
  • To identify common GNB pathogens and their antibiotic susceptibility.
  • To compare outcomes between multidrug-resistant (MDR) and non-MDR GNB infections.

Main Methods:

  • A prospective observational study was conducted in two PICUs over one year.
  • Data collected included patient demographics, isolated organisms, antibiotic susceptibility, and patient outcomes.
  • Statistical analysis was performed to compare outcomes between MDR and non-MDR groups.

Main Results:

  • Out of 1420 PICU admissions, 291 (20.5%) developed GNB infections.
  • The overall mortality rate for GNB infections was 37.1%.
  • Klebsiella was the most common GNB isolated (36.0%), primarily from blood cultures (86.6%).
  • Multidrug-resistant (MDR) GNB infections were identified in one patient, leading to a significantly longer hospital stay (25 days vs. 15 days) compared to non-MDR infections (P < .01).
  • Mortality rates were similar between MDR (37.4%) and non-MDR (35.7%) groups (P = .88).

Conclusions:

  • Pediatric GNB infections, including MDR strains, occur at high rates in PICUs.
  • Effective management requires continuous surveillance systems for GNB infections in critically ill children.
  • Further research into targeted antibiotic strategies is warranted.
Abstract

Keywords:
MDR-GNBhealthcare-associated infectionpediatric intensive care unit

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