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Immunohistochemistry Techniques to Analyze Cellular Proliferation and Neurogenesis in Rats Using the Thymidine Analog BrdU
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A Suzuki-Miyaura method for labelling proliferating cells containing incorporated BrdU

Ning Yan1, Yujun He, Hui Wen

  • 1State Key Laboratory of Bioactive Substances and Function of Natural Medicine, Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, 100050, China. hcui@imm.ac.cn.

The Analyst
|February 13, 2018
PubMed
Summary

A new chemical method using Suzuki-Miyaura coupling allows fluorescent labeling of 5-bromo-2'-deoxyuridine (BrdU) for cell proliferation assays. This approach offers high sensitivity and specificity, overcoming limitations of traditional antibody-based detection.

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