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Multi-analyte Biochip MAB Based on All-solid-state Ion-selective Electrodes ASSISE for Physiological Research
Published on: April 18, 2013
Ion-Selective Electrodes Based on Hydrophilic Ionophore-Modified Nanopores
Soma Papp1, Gyula Jágerszki1, Róbert E Gyurcsányi1
1MTA-BME "Lendület" Chemical Nanosensors Research Group, Department of Inorganic and Analytical Chemistry, Budapest University of Technology and Economics, Szt. Gellért tér 4, 1111, Budapest, Hungary.
Abstract:
We report the synthesis and analytical application of the first Cu2+ -selective synthetic ion channel based on peptide-modified gold nanopores. A Cu2+ -binding peptide motif (Gly-Gly-His) along with two additional functional thiol derivatives inferring cation-permselectivity and hydrophobicity was self-assembled on the surface of gold nanoporous membranes comprising of about 5 nm diameter pores. These membranes were used to construct ion-selective electrodes (ISEs) with extraordinary Cu2+ selectivities, approaching six orders of magnitude over certain ions. Since all constituents are immobilized to a supporting nanoporous membrane, their leaching, that is a ubiquitous problem of conventional ionophore-based ISEs was effectively suppressed.
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