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Updated: Feb 14, 2026

Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects
Published on: February 18, 2014
Measuring Interfacial Polymerization Kinetics Using Microfluidic Interferometry
Arash Nowbahar1, Vincent Mansard2, Jodi M Mecca3
1Department of Chemical Engineering , University of California , Santa Barbara , California 93106 , United States.
Abstract:
A range of academic and industrial fields exploit interfacial polymerization in producing fibers, capsules, and films. Although widely used, measurements of reaction kinetics remain challenging and rarely reported, due to film thinness and reaction rapidity. Here, polyamide film formation is studied using microfluidic interferometry, measuring monomer concentration profiles near the interface during the reaction. Our results reveal that the reaction is initially controlled by a reaction-diffusion boundary layer within the organic phase, which allows the first measurements of the rate constant for this system.
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