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Optical Clearing of Plant Tissues for Fluorescence Imaging
Published on: January 5, 2022
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Recent progress in tissue optical clearing for spectroscopic application.
A Yu Sdobnov1, M E Darvin2, E A Genina3
1Faculty of Information Technology and Electrical Engineering, University of Oulu, Oulu 90570, Finland; Research-Educational Institute of Optics and Biophotonics, Saratov State University (National Research University of Russia), Astrakhanskaya 83, 410012 Saratov, Russian Federation.
Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
|February 14, 2018
Summary
Optical clearing agents reduce scattering in biological tissues, enhancing deep imaging and contrast for various optical methods. This technique improves diagnostics and laser treatments for skin diseases and tumors.
Area of Science:
- Biomedical Optics
- Biophotonics
- Medical Imaging
Background:
- Biological tissues scatter light, limiting optical imaging depth and resolution.
- Optical clearing aims to reduce this scattering by altering tissue optical properties.
Purpose of the Study:
- To review recent advancements in optical clearing of skin and biological tissues.
- To discuss the principles, safety, advantages, and limitations of optical clearing.
- To highlight applications in various optical imaging modalities and clinical practices.
Main Methods:
- Review of literature on optical clearing techniques.
- Application of optical clearing agents in vivo and in vitro.
- Integration with imaging methods like multiphoton microscopy, confocal Raman microscopy, optical coherence tomography, and laser speckle contrast imaging.
Main Results:
- Optical clearing effectively reduces tissue scattering.
- Improved imaging depth, resolution, and contrast are achieved.
- Enhanced visualization of deep-seated tissue structures.
Conclusions:
- Optical clearing significantly benefits optical imaging of biological tissues.
- It enables advanced diagnostics and laser-based treatments for various medical conditions.
- The technique holds promise for clinical applications in dermatology and oncology.
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