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Updated: Feb 14, 2026

Construction of Modular Hydrogel Sheets for Micropatterned Macro-scaled 3D Cellular Architecture
Published on: January 11, 2016
Simple Formation of Cell Arrays Embedded in Hydrogel Sheets and Cubes
Takatomo Sugano1, Yui Sasaki1, Fumio Mizutani1
1Graduate School of Material Science, University of Hyogo.
Abstract:
Arrays with cell aggregations and single-cell arrays embedded in hydrogel sheets were fabricated by negative dielectrophoretic (n-DEP) cell-manipulation techniques and hydrogel gelation. Cells suspended randomly in a prepolymer solution were rapidly manipulated to form an island-like organization of cells through the repulsive force of n-DEP by using a DEP device consisting of grid electrodes. The cell patterns were retained by irradiating ultraviolet (UV) light so as to urge gelation. Moreover, control of the optical transparency of the grid electrode allows for the fabrication of cubes with single cells and cell aggregation.
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